Literature DB >> 14612236

Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD.

Alexandra L Perry1, Tony Worthington, Anthony C Hilton, Peter A Lambert, Alistair J Stirling, Tom S J Elliott.   

Abstract

Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists.

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Year:  2003        PMID： 14612236     DOI： 10.1016/S0378-1097(03)00720-1

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  6 in total

1.  Multiplex touchdown PCR for rapid typing of the opportunistic pathogen Propionibacterium acnes.

Authors:  Emma Barnard; István Nagy; Judit Hunyadkürti; Sheila Patrick; Andrew McDowell
Journal:  J Clin Microbiol       Date:  2015-01-28       Impact factor: 5.948

2.  Propionibacterium acnes types I and II represent phylogenetically distinct groups.

Authors:  Andrew McDowell; Susanna Valanne; Gordon Ramage; Michael M Tunney; Josephine V Glenn; Gregory C McLorinan; Ajay Bhatia; Jean-Francois Maisonneuve; Michael Lodes; David H Persing; Sheila Patrick
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

3.  Emendation of Propionibacterium acnes subsp. acnes (Deiko et al. 2015) and proposal of Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov.

Authors:  Andrew McDowell; Emma Barnard; Jared Liu; Huiying Li; Sheila Patrick
Journal:  Int J Syst Evol Microbiol       Date:  2016-09-25       Impact factor: 2.747

4.  Propionibacterium acnes Susceptibility and Correlation with Hemolytic Phenotype.

Authors:  Travis E Wright; K Keely Boyle; Thomas R Duquin; John K Crane
Journal:  Infect Dis (Auckl)       Date:  2016-10-09

Review 5.  Over a Decade of recA and tly Gene Sequence Typing of the Skin Bacterium Propionibacterium acnes: What Have We Learnt?

Authors:  Andrew McDowell
Journal:  Microorganisms       Date:  2017-12-21

Review 6.  Cutibacterium acnes as an Opportunistic Pathogen: An Update of Its Virulence-Associated Factors.

Authors:  Constance Mayslich; Philippe Alain Grange; Nicolas Dupin
Journal:  Microorganisms       Date:  2021-02-02
  6 in total

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