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Literature DB >> 14607

Simultaneous determination of skeletal muscle fiber, types I, IIA, and IIB by histochemistry.

Abstract

We describe a simplified technique for the histochemical determination of three fiber types from a single section of skeletal muscle. Preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (ATPase) incubation clearly differentiates type I, type IIA, and IIB fibers in human, rat, rabbit, and porcine muscle. In addition, glycine-formaldehyde-calcium preincubation offers better preservation of cytoarchitecture and standardization of incubation time.

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Year: 1977 PMID： 14607 DOI： 10.1001/archneur.1977.00500150057011

Source DB: PubMed Journal: Arch Neurol ISSN： 0003-9942

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Cited

17 in total

Review 9. Pathology of skeletal muscle: principles of reaction patterns and histochemistry and experience with 195 biopsies.

Authors: H J Manz
Journal: Virchows Arch A Pathol Anat Histol Date: 1980

10. Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle.

Authors: Paula Vickerton; Jonathan C Jarvis; James A Gallagher; Riaz Akhtar; Hazel Sutherland; Nathan Jeffery
Journal: Proc Biol Sci Date: 2014-08-07 Impact factor: 5.349

Simultaneous determination of skeletal muscle fiber, types I, IIA, and IIB by histochemistry.

1. Course of denervation atrophy in type I and type II fibres of rat extensor digitorum longus muscle.

2. Cytochemical approaches to the localization of specific adenosine triphosphatases.

3. Adaptive conditioning of skeletal muscle in a large animal model (Sus domesticus).

4. Variable pH dependence of the myosin-ATPase in different muscles of the rat.

5. Oxidative enzymes and myosin-ATPase in the trunk musculature of the river lamprey (Lampetra fluviatilis).

6. Changes in fibre type, number and diameter in developing and ageing skeletal muscle.

7. Effects of ageing and chronic dietary restriction on the morphology of fast and slow muscles of the rat.

8. Simultaneous histochemical demonstration of capillaries and muscle fibre types.

Review 9. Pathology of skeletal muscle: principles of reaction patterns and histochemistry and experience with 195 biopsies.

10. Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle.