| Literature DB >> 14604550 |
Annie H Shinn1, Normita C Bravo, Holden T Maecker, James W Smith.
Abstract
This study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax antigen. TNF-alpha and IFN-gamma production from memory CD4+ T cells were measured and compared to a control group who never received the anthrax vaccine. The optimal antigen concentration for TNF-alpha was determined to be around 7.5 microg/ml and IFN-gamma production was not detected. This assay will be used in future larger prospective studies to further evaluate the cellular immune responses induced by the anthrax vaccine.Entities:
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Year: 2003 PMID： 14604550 DOI： 10.1016/j.jim.2003.07.012
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303