Yong He1, Jun Zhou, Ke-Feng Dou, Yong Chen. 1. Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. heyong007@yahoo.com
Abstract
OBJECTIVE: To develop a surgical model of acute hepatic failure. METHODS: Hepatectomy was performed in rats according to the method described by Higgins and Anderson. Ninety percent liver resection (removing the left lateral, median, and right lateral lobes) (n=7) and 95% liver resection leaving only half of the caudate lobe (n=13) were performed. Hypoglycemia was corrected by giving 20% glucose in the drinking water, coupled with repeated intraperitoneal injection of 5% glucose adapted to glycemia. While the survival rate, alanine transaminase (ALT) and bilirubin were observed. RESULTS: 95% liver resection decreased survival rates of the rats from 86% (90% liver resection) to 23% (P<0.05), and increased bilirubin levels (4.04+/-2.84 mg/dL vs. 1.25+/-1.85 mg/dL [90% liver resection]). CONCLUSION: 95% liver resection is a good rat model for acute hepatic failure.
OBJECTIVE: To develop a surgical model of acute hepatic failure. METHODS: Hepatectomy was performed in rats according to the method described by Higgins and Anderson. Ninety percent liver resection (removing the left lateral, median, and right lateral lobes) (n=7) and 95% liver resection leaving only half of the caudate lobe (n=13) were performed. Hypoglycemia was corrected by giving 20% glucose in the drinking water, coupled with repeated intraperitoneal injection of 5% glucose adapted to glycemia. While the survival rate, alanine transaminase (ALT) and bilirubin were observed. RESULTS: 95% liver resection decreased survival rates of the rats from 86% (90% liver resection) to 23% (P<0.05), and increased bilirubin levels (4.04+/-2.84 mg/dL vs. 1.25+/-1.85 mg/dL [90% liver resection]). CONCLUSION: 95% liver resection is a good rat model for acute hepatic failure.
Authors: Marek Saracyn; Robert Zdanowski; Marek Brytan; Grzegorz Kade; Zbigniew Nowak; Janusz Patera; Przemysław Dyrla; Jerzy Gil; Zofia Wańkowicz Journal: Med Sci Monit Date: 2015-05-22