| Literature DB >> 14599680 |
Genet Mekuria1, Sunita A Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins, Margaret Sedgley.
Abstract
A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.Entities:
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Year: 2003 PMID： 14599680 DOI： 10.1016/j.jviromet.2003.08.014
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014