Literature DB >> 14597520

Polymerase chain reaction detection of Neisseria meningitidis in the intraocular fluid of a patient with endogenous endophthalmitis but without associated meningitis.

Frank T Kerkhoff1, Anneke van der Zee, Anneke M C Bergmans, Aniki Rothova.   

Abstract

PURPOSE: To report a patient with Neisseria meningitidis endophthalmitis without associated meningitis with full visual recovery, with early detection of the microorganism using polymerase chain reaction (PCR) analysis.
DESIGN: Retrospective, observational case report. PARTICIPANTS: One patient with endogenous endophthalmitis.
METHODS: Polymerase chain reaction analysis and culture of the intraocular fluid sample. Polymerase chain reaction analysis was performed with a universal (16S rRNA) primer set to detect bacterial DNA, and subsequently a specific probe was used to detect Neisseria species DNA.
RESULTS: The 16S rRNA primers detected bacterial DNA, the specific probe detected Neisseria species DNA, and culture was positive for Neisseria meningitidis serotype C.
CONCLUSIONS: A universal bacterial PCR can be very helpful for the diagnosis of endogenous bacterial endophthalmitis at an early stage of the disease.

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Year:  2003        PMID: 14597520     DOI: 10.1016/S0161-6420(03)00834-0

Source DB:  PubMed          Journal:  Ophthalmology        ISSN: 0161-6420            Impact factor:   12.079


  4 in total

1.  Polymerase chain reaction in intraocular inflammation.

Authors:  Krishnendu Nandi; Prabhat Ranjan; Lily Therese; Jyotirmay Biswas
Journal:  Open Ophthalmol J       Date:  2008-10-22

2.  Endogenous endophthalmitis secondary to bacterial meningitis from Neisseria Meningitidis: a case report and review of the literature.

Authors:  Konstantinos Balaskas; Dora Potamitou
Journal:  Cases J       Date:  2009-10-07

3.  Bilateral endophthalmitis as the initial presentation of bacterial meningitis.

Authors:  Seong-Jae Kim; Seong Wook Seo; Jong-Moon Park; In-Young Chung
Journal:  Korean J Ophthalmol       Date:  2009-12-04

Review 4.  Atypical, Yet Not Infrequent, Infections with Neisseria Species.

Authors:  Maria Victoria Humbert; Myron Christodoulides
Journal:  Pathogens       Date:  2019-12-20
  4 in total

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