Alzheimer's disease beta-amyloid precursor protein in rat neural cells in culture.

Immunochemical studies were performed on Alzheimer's beta-amyloid precursor protein (APP) in rat brain and cultured rat neural cells. Multiple APP subtypes were detected on immunoblots of brain homogenate with several antisera specific for subsequences of APP. In rat neural cell cultures, it was demonstrated that the composition of APP subtypes differed among cell types and subcellular fractions, and that APP subtypes in PC12h cells varied in their heparin binding affinity, suggesting distinct functional roles for different APP subtypes. Compatible with the possible role of APP in cell-matrix interaction, an increase in oligodendroglial APP was observed following their attachment onto poly-L-lysine substratum.