Literature DB >> 14592524

An efficient enzymatic synthesis of thiamin pyrophosphate.

Jonathan S Melnick1, K Ingrid Sprinz, Jason J Reddick, Cynthia Kinsland, Tadhg P Begley.   

Abstract

Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.

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Year:  2003        PMID： 14592524     DOI： 10.1016/j.bmcl.2003.07.026

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  5 in total

1.  Solid-state NMR and density functional theory studies of ionization states of thiamin.

Authors:  Sivakumar Paramasivam; Anand Balakrishnan; Olga Dmitrenko; Amy Godert; Tadhg P Begley; Frank Jordan; Tatyana Polenova
Journal:  J Phys Chem B       Date:  2010-12-22       Impact factor: 2.991

2.  EPR spectroscopic and computational characterization of the hydroxyethylidene-thiamine pyrophosphate radical intermediate of pyruvate:ferredoxin oxidoreductase.

Authors:  Steven O Mansoorabadi; Javier Seravalli; Cristina Furdui; Vladimir Krymov; Gary J Gerfen; Tadhg P Begley; Jonathan Melnick; Stephen W Ragsdale; George H Reed
Journal:  Biochemistry       Date:  2006-06-13       Impact factor: 3.162

3.  Pathway engineered enzymatic de novo purine nucleotide synthesis.

Authors:  Heather L Schultheisz; Blair R Szymczyna; Lincoln G Scott; James R Williamson
Journal:  ACS Chem Biol       Date:  2008-08-15       Impact factor: 5.100

4.  Competence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitamin.

Authors:  Natalia S Nemeria; Brateen Shome; Alicia A DeColli; Kathryn Heflin; Tadhg P Begley; Caren Freel Meyers; Frank Jordan
Journal:  Biochemistry       Date:  2016-02-08       Impact factor: 3.162

5.  An enzymatic platform for the synthesis of isoprenoid precursors.

Authors:  Sofia B Rodriguez; Thomas S Leyh
Journal:  PLoS One       Date:  2014-08-25       Impact factor: 3.240
  5 in total

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