Literature DB >> 14587709

Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites.

Michael E Webb1, Elaine Stephens, Alison G Smith, Chris Abell.   

Abstract

The binding affinity of aspartate decarboxylase has been probed using MALDI-TOF spectrometry; adducts formed covalently in the active site were detected by MALDI-TOF mass spectrometry after incubation of the enzyme with a range of potential ligands in the presence of NaCNBH3; this has highighted key structural features which will aid design of potential inhibitors.

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Year:  2003        PMID： 14587709     DOI： 10.1039/b308182f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

1.  Structure of Escherichia coli aspartate α-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation.

Authors:  Michael E Webb; Carina M C Lobley; Fatima Soliman; Mairi L Kilkenny; Alison G Smith; Tom L Blundell; Chris Abell
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-03-28

2.  Validation of drug-like inhibitors against Mycobacterium tuberculosis L-aspartate α-decarboxylase using nuclear magnetic resonance (1H NMR).

Authors:  Reetu Sharma; Mara Florea; Werner M Nau; Kunchithapadam Swaminathan
Journal:  PLoS One       Date:  2012-09-28       Impact factor: 3.240

3.  Chemoinformatic identification of novel inhibitors against Mycobacterium tuberculosis L-aspartate α-decarboxylase.

Authors:  Reetu Sharma; Roopa Kothapalli; Antonius M J Van Dongen; Kunchithapadam Swaminathan
Journal:  PLoS One       Date:  2012-03-28       Impact factor: 3.240

4.  Modular Enzymatic Cascade Synthesis of Vitamin B5 and Its Derivatives.

Authors:  Mohammad Z Abidin; Thangavelu Saravanan; Jielin Zhang; Pieter G Tepper; Erick Strauss; Gerrit J Poelarends
Journal:  Chemistry       Date:  2018-10-30       Impact factor: 5.236

5.  Confirmation of a Protein-Protein Interaction in the Pantothenate Biosynthetic Pathway by Using Sortase-Mediated Labelling.

Authors:  Philip M Morrison; Matthew R Balmforth; Samuel W Ness; Daniel J Williamson; Michael D Rugen; W Bruce Turnbull; Michael E Webb
Journal:  Chembiochem       Date:  2016-03-01       Impact factor: 3.164

6.  Threonine 57 is required for the post-translational activation of Escherichia coli aspartate α-decarboxylase.

Authors:  Michael E Webb; Briony A Yorke; Tom Kershaw; Sarah Lovelock; Carina M C Lobley; Mairi L Kilkenny; Alison G Smith; Tom L Blundell; Arwen R Pearson; Chris Abell
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2014-03-21
  6 in total

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