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Literature DB >> 14586552

Development of a novel Agrobacterium-mediated transformation method to recover transgenic Brassica napus plants.

Abstract

We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treatment or by high density planting. Molecular and genetic analysis revealed that single and multiple copy events were generated and that the transgenes were transmitted to the T1 and T2 progeny in a Mendelian fashion.

Entities: Species

Mesh：

Substances：
DNA Primers

Year: 2003 PMID： 14586552 DOI： 10.1007/s00299-003-0691-9

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

11 in total

1. Recent advances in the transformation of plants.

Authors:
Journal: Trends Plant Sci Date: 1999-06 Impact factor: 18.313

2. Floral spray transformation can efficiently generate Arabidopsis transgenic plants.

Authors: M H Chung; M K Chen; S M Pan
Journal: Transgenic Res Date: 2000-12 Impact factor: 2.788

3. High efficiency transformation ofBrassica napus usingAgrobacterium vectors.

Authors: M M Moloney; J M Walker; K K Sharma
Journal: Plant Cell Rep Date: 1989-04 Impact factor: 4.570

4. Transgenic radish (Raphanus sativus L. longipinnatus Bailey) by floral-dip method--plant development and surfactant are important in optimizing transformation efficiency.

Authors: I S Curtis; H G Nam
Journal: Transgenic Res Date: 2001-08 Impact factor: 2.788

5. The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana.

Authors: N Bechtold; B Jaudeau; S Jolivet; B Maba; D Vezon; R Voisin; G Pelletier
Journal: Genetics Date: 2000-08 Impact factor: 4.562

6. Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method.

Authors: C Desfeux; S J Clough; A F Bent
Journal: Plant Physiol Date: 2000-07 Impact factor: 8.340

7. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation.

Authors: G N Ye; D Stone; S Z Pang; W Creely; K Gonzalez; M Hinchee
Journal: Plant J Date: 1999-08 Impact factor: 6.417

8. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

Authors: S J Clough; A F Bent
Journal: Plant J Date: 1998-12 Impact factor: 6.417

9. Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation.

Authors: G Vancanneyt; R Schmidt; A O'Connor-Sanchez; L Willmitzer; M Rocha-Sosa
Journal: Mol Gen Genet Date: 1990-01

10. Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation.

Authors: S J Rothstein; K N Lahners; R J Lotstein; N B Carozzi; S M Jayne; D A Rice
Journal: Gene Date: 1987 Impact factor: 3.688

8 in total

1. Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.

Authors: Jianzhong Lin; Bo Zhou; Yuanzhu Yang; Jin Mei; Xiaoying Zhao; Xinhong Guo; Xingqun Huang; Dongying Tang; Xuanming Liu
Journal: Plant Cell Rep Date: 2009-05-20 Impact factor: 4.570

2. Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules.

Authors: Inger Baeksted Holme; Henrik Brinch-Pedersen; Mette Lange; Preben Bach Holm
Journal: Plant Cell Rep Date: 2006-07-11 Impact factor: 4.570

3. A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B.

Authors: Wei Hu; J Clark Lagarias
Journal: Plant Physiol Date: 2016-11-23 Impact factor: 8.340

4. Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration.

Authors: Kondeti Subramanyam; Koona Subramanyam; K V Sailaja; M Srinivasulu; K Lakshmidevi
Journal: Plant Cell Rep Date: 2011-01-07 Impact factor: 4.570