Literature DB >> 14586551

A novel selection system for potato transformation using a mutated AHAS gene.

M Andersson1, A Trifonova, A-B Andersson, M Johansson, L Bülow, P Hofvander.   

Abstract

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a beta-glucuronidase (GUS) gene. Selection on 0.5 microM Imazamox yielded GUS expression in 93-100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 14586551     DOI： 10.1007/s00299-003-0684-8

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  17 in total

1.  A chimaeric hygromycin resistance gene as a selectable marker in plant cells.

Authors:  P J van den Elzen; J Townsend; K Y Lee; J R Bedbrook
Journal:  Plant Mol Biol       Date:  1985-09       Impact factor: 4.076

2.  Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs.

Authors:  R G Visser; I Somhorst; G J Kuipers; N J Ruys; W J Feenstra; E Jacobsen
Journal:  Mol Gen Genet       Date:  1991-02

3.  Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants.

Authors:  T B Ray
Journal:  Plant Physiol       Date:  1984-07       Impact factor: 8.340

4.  Engineering herbicide tolerance in transgenic plants.

Authors:  D M Shah; R B Horsch; H J Klee; G M Kishore; J A Winter; N E Tumer; C M Hironaka; P R Sanders; C S Gasser; S Aykent; N R Siegel; S G Rogers; R T Fraley
Journal:  Science       Date:  1986-07-25       Impact factor: 47.728

5.  Regulation of tobacco acetolactate synthase gene expression.

Authors:  S J Keeler; P Sanders; J K Smith; B J Mazur
Journal:  Plant Physiol       Date:  1993-07       Impact factor: 8.340

6.  New plant binary vectors with selectable markers located proximal to the left T-DNA border.

Authors:  D Becker; E Kemper; J Schell; R Masterson
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

7.  Agrobacterium-mediated transformation of Solanum tuberosum L. cv. 'Russet Burbank'.

Authors:  C A Newell; R Rozman; M A Hinchee; E C Lawson; L Haley; P Sanders; W Kaniewski; N E Tumer; R B Horsch; R T Fraley
Journal:  Plant Cell Rep       Date:  1991-05       Impact factor: 4.570

8.  Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium.

Authors:  G Ooms; M M Burrell; A Karp; M Bevan; J Hille
Journal:  Theor Appl Genet       Date:  1987-09       Impact factor: 5.699

9.  Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments.

Authors:  R G Visser; E Jacobsen; A Hesseling-Meinders; M J Schans; B Witholt; W J Feenstra
Journal:  Plant Mol Biol       Date:  1989-03       Impact factor: 4.076

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
View more
  15 in total

1.  Evaluation of selection strategies alternative to nptII in genetic transformation of citrus.

Authors:  Alida Ballester; Magdalena Cervera; Leandro Peña
Journal:  Plant Cell Rep       Date:  2008-03-04       Impact factor: 4.570

2.  Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015.

Authors:  Marit Lenman; Ashfaq Ali; Per Mühlenbock; Ulrika Carlson-Nilsson; Erland Liljeroth; Nicolas Champouret; Vivianne G A A Vleeshouwers; Erik Andreasson
Journal:  Theor Appl Genet       Date:  2015-10-30       Impact factor: 5.699

3.  Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese.

Authors:  Chang Kil Kim; Jeung-Sul Han; Hyun-Suk Lee; Joung-Youl Oh; Thoshiro Shigaki; Sung Hun Park; Kendal Hirschi
Journal:  Plant Cell Rep       Date:  2006-10-06       Impact factor: 4.570

4.  Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene.

Authors:  Yojiro Taniguchi; Motoshige Kawata; Ikuo Ando; Tsutomu Shimizu; Masahiro Ohshima
Journal:  Plant Cell Rep       Date:  2010-09-03       Impact factor: 4.570

5.  A novel mutated acetolactate synthase gene conferring specific resistance to pyrimidinyl carboxy herbicides in rice.

Authors:  Ayako Okuzaki; Tsutomu Shimizu; Koichiro Kaku; Kiyoshi Kawai; Kinya Toriyama
Journal:  Plant Mol Biol       Date:  2007-03-03       Impact factor: 4.076

6.  Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker.

Authors:  Taiichi Ogawa; Hiroyuki Kawahigashi; Seiichi Toki; Hirokazu Handa
Journal:  Plant Cell Rep       Date:  2008-05-01       Impact factor: 4.570

7.  The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.

Authors:  Florian Veillet; Laura Chauvin; Marie-Paule Kermarrec; François Sevestre; Mathilde Merrer; Zoé Terret; Nicolas Szydlowski; Pierre Devaux; Jean-Luc Gallois; Jean-Eric Chauvin
Journal:  Plant Cell Rep       Date:  2019-05-17       Impact factor: 4.570

8.  Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism.

Authors:  Per Hofvander; Till Ischebeck; Helle Turesson; Sandeep K Kushwaha; Ivo Feussner; Anders S Carlsson; Mariette Andersson
Journal:  Plant Biotechnol J       Date:  2016-03-17       Impact factor: 9.803

9.  Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.

Authors:  Adrienne Forsyth; Troy Weeks; Craig Richael; Hui Duan
Journal:  Front Plant Sci       Date:  2016-10-25       Impact factor: 5.753

10.  Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts.

Authors:  Mariette Andersson; Helle Turesson; Alessandro Nicolia; Ann-Sofie Fält; Mathias Samuelsson; Per Hofvander
Journal:  Plant Cell Rep       Date:  2016-10-03       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.