Literature DB >> 1457970

Structure elucidation of sulphated oligosaccharides from recombinant human tissue plasminogen activator expressed in mouse epithelial cells.

G Pfeiffer1, S Stirm, R Geyer, K H Strube, A A Bergwerff, J P Kamerling, J F Vliegenthart.   

Abstract

Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAc-alpha 2-6Gal beta 1-4GlcNAc beta 1-2[NeuAc alpha 2-3Gal beta 1- 4GlcNAc beta 1-4]-Man alpha 1-3[NeuAc alpha 2-3(SO4-6)Gal beta 1- 4-GlcNAc beta 1-2Man alpha 1-6]-Man beta 1-4GlcNAc beta 1- 4[Fuc alpha 1-6]GlcNAc-o1.

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Year:  1992        PMID： 1457970     DOI： 10.1093/glycob/2.5.411

Source DB:  PubMed          Journal:  Glycobiology        ISSN： 0959-6658            Impact factor:   4.313


  5 in total

1.  Characterization of a spleen sulphotransferase responsible for the 6-O-sulphation of the galactose residue in sialyl-N-acetyl-lactosamine sequences.

Authors:  R G Spiro; V D Bhoyroo
Journal:  Biochem J       Date:  1998-04-01       Impact factor: 3.857

2.  The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans.

Authors:  A J Jaques; G Opdenakker; T W Rademacher; R A Dwek; S E Zamze
Journal:  Biochem J       Date:  1996-06-01       Impact factor: 3.857

3.  The structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage: oligosaccharides generated by keratanase II digestion.

Authors:  R M Lauder; T N Huckerby; I A Nieduszynski
Journal:  Glycoconj J       Date:  1995-10       Impact factor: 2.916

Review 4.  Analysis of protein glycosylation by mass spectrometry.

Authors:  B Nilsson
Journal:  Mol Biotechnol       Date:  1994-12       Impact factor: 2.695

5.  A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis.

Authors:  Jing-Rong Wang; Wei-Na Gao; Rudolf Grimm; Shibo Jiang; Yong Liang; Hua Ye; Zhan-Guo Li; Lee-Fong Yau; Hao Huang; Ju Liu; Min Jiang; Qiong Meng; Tian-Tian Tong; Hai-Hui Huang; Stephanie Lee; Xing Zeng; Liang Liu; Zhi-Hong Jiang
Journal:  Nat Commun       Date:  2017-09-20       Impact factor: 14.919
  5 in total

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