Literature DB >> 1457816

M13 single-strand purification using a biotinylated probe and streptavidin coated magnetic beads.

T Hawkins1.   

Abstract

A new method for M13 single-strand purification has been developed and tested. The procedure gives sufficient DNA for one set of thermally cycled sequencing reactions when analysed on the Applied Biosystems 373A fluorescent DNA sequencer. For this report, 250 random M13 subclones with 1-2 Kb inserts were grown and prepared using this method.

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Year:  1992        PMID： 1457816     DOI： 10.3109/10425179209033998

Source DB:  PubMed          Journal:  DNA Seq        ISSN： 1026-7913


  4 in total

1.  Large-scale production of DNA sequencing templates by microtitre format PCR.

Authors:  A Rosenthal; O Coutelle; M Craxton
Journal:  Nucleic Acids Res       Date:  1993-01-11       Impact factor: 16.971

2.  Solid-phase reversible immobilization for the isolation of PCR products.

Authors:  M M DeAngelis; D G Wang; T L Hawkins
Journal:  Nucleic Acids Res       Date:  1995-11-25       Impact factor: 16.971

3.  High-throughput detergent extraction of M13 subclones for fluorescent DNA sequencing.

Authors:  E R Mardis
Journal:  Nucleic Acids Res       Date:  1994-06-11       Impact factor: 16.971

4.  DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry.

Authors:  J R Edwards; Y Itagaki; J Ju
Journal:  Nucleic Acids Res       Date:  2001-11-01       Impact factor: 16.971
  4 in total

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