Differential responses of HSPs to heat stress in slow and fast regions of rat gastrocnemius muscle.

In a recent study, we showed that the rat slow soleus and fast plantaris muscles exhibited different time courses for the response of specific heat shock proteins (HSPs) after 1 h of heat stress. We hypothesized that these differential responses were related, in part, to the varying fiber type composition of these muscles. To further test this hypothesis, we now have determined the responses of Hsp60, Hsp72, and Hsc73 during the 60 h following exposure to a single bout of heat stress in the deep (relatively high percentage of slow fibers) and superficial regions (only fast fibers) of the adult rat gastrocnemius muscle. The temperature of the musculature in the left hindlimb was elevated to approximately 42 degrees C for 1 h, while the right hindlimb served as a control. Two hours after the heat stress, the Hsp60 levels were increased by 1.3- and 2.0-fold in the deep and superficial regions, respectively. The Hsp72 levels were increased (1.8-fold) in the deep region at 8 h after heat stress, whereas in the superficial region these levels were increased between 4 and 48 h (peak at 36 h by 10-fold) after the heat stress. No changes were observed for Hsc73 in either region of the muscle. Combined with our previous data, the results indicate that the responses of HSPs in the rat hindlimb muscles after a single exposure to heat stress are related to fiber type composition of the muscle or muscle region or to the inherent properties of each HSP. From a clinical viewpoint, these data indicate that specific regions (most likely based on fiber type composition) within a muscle may be affected differentially by any intervention inducing HSPs.