| Literature DB >> 1456956 |
C R Knudsen1, B F Clark, B Degn, O Wiborg.
Abstract
The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa.Entities:
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Year: 1992 PMID： 1456956
Source DB: PubMed Journal: Biochem Int ISSN： 0158-5231