| Literature DB >> 14568073 |
Catherine M Kramer1, Rogerio T N Prata, Michael G Willits, Vincenzo De Luca, John C Steffens, Gerson Graser.
Abstract
Two UDP-glucose-dependent flavonoid glucosyltransferases (EC 2.4.1.-) isolated from the epidermal layer of yellow onion (Allium cepa) were functionally expressed in Escherichia coli and their substrate specificity investigated. The two enzymes exhibited different substrate- and regio-specificity profiles. A. cepa UGT73G1 used a wide range of different flavonoid substrates including flavonoids not naturally occurring in onion. Regiospecificity was indicated for hydroxyl-groups of the C-3, C-7 and C-4' positions of the flavan backbone structure to yield flavonoid mono- and diglucosides. In contrast, A. cepa UGT73J1 showed activity only with the flavonoid mono-glucoside isoquercitrin and the isoflavone aglycone genistein, with regiospecificity for the C-7 position. The regiospecificity for both enzymes included positions that are glucosylated in flavonoids of onion bulbs, indicating their involvement in flavonoid biosynthesis in A. cepa.Entities:
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Year: 2003 PMID： 14568073 DOI： 10.1016/s0031-9422(03)00507-7
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072