| Literature DB >> 14565470 |
T Wenzel1, T Elssner, K Fahr, J Bimmler, S Richter, I Thomas, M Kostrzewa.
Abstract
A photocleavable o-nitrobenzyl CE phosphoramidite building-block was synthesised and incorporated within oligonucleotides. After allele-specific primer extension, desalting was performed using genostrep purification plates. Release of the SNP information containing part through photocleavage created shortened molecules that are easily accessible for MALDI-TOF analysis. Additionally, incorporation of mass modified nucleosides enables flexible design of multiplex genotyping.Entities:
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Year: 2003 PMID： 14565470 DOI： 10.1081/NCN-120023038
Source DB: PubMed Journal: Nucleosides Nucleotides Nucleic Acids ISSN： 1525-7770 Impact factor: 1.381