C J Lee1, B R Do, J K Kim, Y D Yoon. 1. Korea Atomic Energy Research Institute, Yusong PO Box 105, Taejon 305-600, Korea.
Abstract
PURPOSE: To investigate the potential effects of pentobarbital and ketamine on serum concentrations of sex hormones, the present study was performed using pregnant mare serum gonadotropin (PMSG)-primed cyclic female Sprague-Dawley rats. METHODS: Pentobarbital sodium (37 mg.kg(-1), i. p.) or ketamine-hydrochloride (229 mg.kg(-1), i. m.) was injected 2 and 3 days after PMSG treatment. At 0, 1, 2, 3, 4, and 5 days after PMSG treatment, sera were collected by cardiac puncture. The serum concentrations of progesterone (P(4)), testosterone (T), and estradiol-17 beta (E(2)) were determined by radioimmunoassay. RESULTS: The serum concentrations of P(4) tended to be lower in both the pentobarbital- and the ketamine-treated groups compared with the control group. Significant differences were found on days 3 and 4 after pentobarbital and on days, 1, 4, and 5 after ketamine administration. Serum concentrations of T were also suppressed in both the pentobarbital and the ketamine-treated groups, whereas E(2) concentrations decreased only in the ketamine-treated group. CONCLUSION: Pentobarbital and ketamine decrease serum sex hormone concentrations in PMSG-primed female rats.
PURPOSE: To investigate the potential effects of pentobarbital and ketamine on serum concentrations of sex hormones, the present study was performed using pregnant mare serum gonadotropin (PMSG)-primed cyclic female Sprague-Dawley rats. METHODS:Pentobarbital sodium (37 mg.kg(-1), i. p.) or ketamine-hydrochloride (229 mg.kg(-1), i. m.) was injected 2 and 3 days after PMSG treatment. At 0, 1, 2, 3, 4, and 5 days after PMSG treatment, sera were collected by cardiac puncture. The serum concentrations of progesterone (P(4)), testosterone (T), and estradiol-17 beta (E(2)) were determined by radioimmunoassay. RESULTS: The serum concentrations of P(4) tended to be lower in both the pentobarbital- and the ketamine-treated groups compared with the control group. Significant differences were found on days 3 and 4 after pentobarbital and on days, 1, 4, and 5 after ketamine administration. Serum concentrations of T were also suppressed in both the pentobarbital and the ketamine-treated groups, whereas E(2) concentrations decreased only in the ketamine-treated group. CONCLUSION:Pentobarbital and ketamine decrease serum sex hormone concentrations in PMSG-primed female rats.
Authors: Anna A Rybczynska; Philip H Elsinga; Jurgen W Sijbesma; Kiichi Ishiwata; Johan R de Jong; Erik F de Vries; Rudi A Dierckx; Aren van Waarde Journal: Eur J Nucl Med Mol Imaging Date: 2009-02-27 Impact factor: 9.236