OBJECTIVE: The purpose of this study was to quantify the cytokines interleukin (IL)-1 receptor antagonist (RA), IL-6, and transforming growth factor beta(1) found in extracts of inflammatory periapical tissues and to study the effect of a new antibiotic (linezolid) on the levels of these cytokines. STUDY DESIGN:Twenty-two patients with root-filled teeth with persisting periapical pathoses were randomly divided into a control group or an antibiotic group. One tooth from each of the patients was resected at the root-end, and the periapical tissue was collected. IL-1RA, IL-6, and TGF-beta(1) were quantified in tissue extracts through the use of enzyme-linked immunosorbent assays. RESULTS: Measurable amounts of all 3 cytokines were found in all extracts. A statistically significant reduction in IL-1RA per milliliter was observed in the linezolid group in comparison with the control group (P <.05). The difference in variation of IL-1RA in the treatment and control groups was also highly statistically significant (P <.001). CONCLUSIONS: Although drawn from a limited sample size, the results indicate that IL-1RA is a sensitive indicator of the effect of antibacterial treatment on the immune response in periapical inflammatory tissues.
RCT Entities:
OBJECTIVE: The purpose of this study was to quantify the cytokines interleukin (IL)-1 receptor antagonist (RA), IL-6, and transforming growth factor beta(1) found in extracts of inflammatory periapical tissues and to study the effect of a new antibiotic (linezolid) on the levels of these cytokines. STUDY DESIGN: Twenty-two patients with root-filled teeth with persisting periapical pathoses were randomly divided into a control group or an antibiotic group. One tooth from each of the patients was resected at the root-end, and the periapical tissue was collected. IL-1RA, IL-6, and TGF-beta(1) were quantified in tissue extracts through the use of enzyme-linked immunosorbent assays. RESULTS: Measurable amounts of all 3 cytokines were found in all extracts. A statistically significant reduction in IL-1RA per milliliter was observed in the linezolid group in comparison with the control group (P <.05). The difference in variation of IL-1RA in the treatment and control groups was also highly statistically significant (P <.001). CONCLUSIONS: Although drawn from a limited sample size, the results indicate that IL-1RA is a sensitive indicator of the effect of antibacterial treatment on the immune response in periapical inflammatory tissues.
Authors: Xinyan Liu; Yaling He; Kun Xiao; Julie R White; Dahlene N Fusco; Genovefa A Papanicolaou Journal: PLoS One Date: 2013-03-05 Impact factor: 3.240
Authors: Stephen J Evans; Aled E L Roberts; Andrew Conway Morris; A John Simpson; Llinos G Harris; Dietrich Mack; Rowena E Jenkins; Thomas S Wilkinson Journal: Sci Rep Date: 2020-10-02 Impact factor: 4.379