Literature DB >> 14559214

Construction of a gorilla fosmid library and its PCR screening system.

Choong Gon Kim1, Asao Fujiyama, Naruya Saitou.   

Abstract

A gorilla fosmid library of 261,120 independent clones was constructed and characterized. The fosmid vector is similar to the cosmid in average insert size of ca. 40 kb but contains the F factor for replication, and it is more resistant to recombination. This clone library represents about 3.7 times coverage of the gorilla genome. A simple screening system by PCR was established, and we successfully found 9 clones that cover the entire Hox A gene cluster of the gorilla genome. This gorilla fosmid DNA library is a useful resource for comparative genomics of human and apes.

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Year:  2003        PMID： 14559214     DOI： 10.1016/s0888-7543(03)00174-5

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  13 in total

1.  Fosmid library construction and initial analysis of end sequences in Zhikong scallop (Chlamys farreri).

Authors:  Lingling Zhang; Zhenmin Bao; Jie Cheng; Hui Li; Xiaoting Huang; Shi Wang; Can Zhang; Jingjie Hu
Journal:  Mar Biotechnol (NY)       Date:  2007-06-29       Impact factor: 3.619

2.  Characterization of rubber tree microRNA in phytohormone response using large genomic DNA libraries, promoter sequence and gene expression analysis.

Authors:  Supanath Kanjanawattanawong; Sithichoke Tangphatsornruang; Kanokporn Triwitayakorn; Panthita Ruang-areerate; Duangjai Sangsrakru; Supannee Poopear; Suthasinee Somyong; Jarunya Narangajavana
Journal:  Mol Genet Genomics       Date:  2014-05-26       Impact factor: 3.291

3.  Random sheared fosmid library as a new genomic tool to accelerate complete finishing of rice (Oryza sativa spp. Nipponbare) genome sequence: sequencing of gap-specific fosmid clones uncovers new euchromatic portions of the genome.

Authors:  Jetty S S Ammiraju; Yeisoo Yu; Meizhong Luo; Dave Kudrna; HyeRan Kim; Jose L Goicoechea; Yuichi Katayose; Takashi Matsumoto; Jianzhong Wu; Takuji Sasaki; Rod A Wing
Journal:  Theor Appl Genet       Date:  2005-11-10       Impact factor: 5.699

4.  Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan.

Authors:  Lixia Wang; Shinji Kikuchi; Chiaki Muto; Ken Naito; Takehisa Isemura; Masao Ishimoto; Xuzhen Cheng; Akito Kaga; Norihiko Tomooka
Journal:  J Plant Res       Date:  2015-03-22       Impact factor: 2.629

5.  Diversity in copy number and structure of a silkworm morphogenetic gene as a result of domestication.

Authors:  Takashi Sakudoh; Takeharu Nakashima; Yoko Kuroki; Asao Fujiyama; Yuji Kohara; Naoko Honda; Hirofumi Fujimoto; Toru Shimada; Masao Nakagaki; Yutaka Banno; Kozo Tsuchida
Journal:  Genetics       Date:  2011-01-17       Impact factor: 4.562

6.  Fosmid library construction and initial analysis of end sequences in female half-smooth tongue sole (Cynoglossus semilaevis).

Authors:  Xubo Wang; Quanqi Zhang; Xiaohua Sun; Yanjie Chen; Teng Zhai; Wei Zhuang; Jie Qi; Zhigang Wang
Journal:  Mar Biotechnol (NY)       Date:  2008-09-02       Impact factor: 3.619

Review 7.  Advances in BAC-based physical mapping and map integration strategies in plants.

Authors:  Ruvini Ariyadasa; Nils Stein
Journal:  J Biomed Biotechnol       Date:  2012-02-27

8.  Analysis of the genome of a Korean isolate of the Pieris rapae granulovirus enabled by its separation from total host genomic DNA by pulse-field electrophoresis.

Authors:  Yong Hun Jo; Bharat Bhusan Patnaik; Se Won Kang; Sung-Hwa Chae; Seunghan Oh; Dong Hyun Kim; Mi Young Noh; Gi Won Seo; Heon Cheon Jeong; Ju Young Noh; Ji Eun Jeong; Hee Ju Hwang; Kisung Ko; Yeon Soo Han; Yong Seok Lee
Journal:  PLoS One       Date:  2013-12-31       Impact factor: 3.240

9.  Sequencing of the core MHC region of black grouse (Tetrao tetrix) and comparative genomics of the galliform MHC.

Authors:  Biao Wang; Robert Ekblom; Tanja M Strand; Silvia Portela-Bens; Jacob Höglund
Journal:  BMC Genomics       Date:  2012-10-15       Impact factor: 3.969

10.  Novel method for high-throughput colony PCR screening in nanoliter-reactors.

Authors:  Marcel Walser; Rene Pellaux; Andreas Meyer; Matthias Bechtold; Herve Vanderschuren; Richard Reinhardt; Joseph Magyar; Sven Panke; Martin Held
Journal:  Nucleic Acids Res       Date:  2009-03-12       Impact factor: 16.971
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