OBJECTIVE: To study the role of follicular melatonin on steroid production by human preovulatory follicles. DESIGN: In vivo comparative and in vitro culture studies. SETTING: University hospital. PATIENT(S): Thirty-six patients with tubal and/or male, but not endocrinological, infertility factors. INTERVENTION(S): Follicular fluid collection during IVF. In vitro granulosa cell culture from luteinizing or growing Graafian follicles. MAIN OUTCOME MEASURE(S): Follicular melatonin, P, T, and E(2) concentrations. In vitro P production by granulosa cells. RESULT(S): There was a positive correlation between follicular melatonin and P concentrations. Melatonin did not stimulate in vitro P production by granulosa cells from luteinizing or growing follicles. Melatonin, P, and E(2) concentrations were significantly higher, but T concentrations were lower, in large follicles than in small follicles. CONCLUSION(S): Preovulatory follicles contain a high amount of melatonin compared with that in small immature follicles; melatonin may play an important, but indirect, role in P production by human granulosa cells.
OBJECTIVE: To study the role of follicular melatonin on steroid production by human preovulatory follicles. DESIGN: In vivo comparative and in vitro culture studies. SETTING: University hospital. PATIENT(S): Thirty-six patients with tubal and/or male, but not endocrinological, infertility factors. INTERVENTION(S): Follicular fluid collection during IVF. In vitro granulosa cell culture from luteinizing or growing Graafian follicles. MAIN OUTCOME MEASURE(S): Follicular melatonin, P, T, and E(2) concentrations. In vitro P production by granulosa cells. RESULT(S): There was a positive correlation between follicular melatonin and P concentrations. Melatonin did not stimulate in vitro P production by granulosa cells from luteinizing or growing follicles. Melatonin, P, and E(2) concentrations were significantly higher, but T concentrations were lower, in large follicles than in small follicles. CONCLUSION(S): Preovulatory follicles contain a high amount of melatonin compared with that in small immature follicles; melatonin may play an important, but indirect, role in P production by human granulosa cells.
Authors: Emerson S Posadas; Charles J Meliska; Luis F Martinez; Diane L Sorenson; Ana M Lopez; Sara Nowakowski; Richard Hauger; B L Parry Journal: J Womens Health (Larchmt) Date: 2012-02-09 Impact factor: 2.681
Authors: Darío Acuña-Castroviejo; Germaine Escames; Carmen Venegas; María E Díaz-Casado; Elena Lima-Cabello; Luis C López; Sergio Rosales-Corral; Dun-Xian Tan; Russel J Reiter Journal: Cell Mol Life Sci Date: 2014-02-20 Impact factor: 9.261