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Literature DB >> 14552769

Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR.

Masayasu Kuwahara¹, Yumi Takahata, Atsushi Shoji, Akiko N Ozaki, Hiroaki Ozaki, Hiroaki Sawai.

Abstract

In order to enhance a collection of modified deoxynucleoside triphosphates useful for in vitro selection or SELEX (systematic evolution of ligands by exponential enrichment) techniques, we designed and synthesized modified analogues of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate bearing a flexible and hydrophilic 7-amino-2,5-dioxaheptyl linker at a C5 position. Both analogues were found to be substrates for thermostable DNA polymerases which belong to an evolutional family B during PCR.

Entities: Chemical

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Year: 2003 PMID： 14552769 DOI： 10.1016/j.bmcl.2003.08.001

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

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Cited

9 in total

1. Substrate specificity and kinetic framework of a DNAzyme with an expanded chemical repertoire: a putative RNaseA mimic that catalyzes RNA hydrolysis independent of a divalent metal cation.

Authors: Richard Ting; Jason M Thomas; Leonard Lermer; David M Perrin
Journal: Nucleic Acids Res Date: 2004-12-29 Impact factor: 16.971

Review 2. In vitro selection of BNA (LNA) aptamers.

Authors: Masayasu Kuwahara; Satoshi Obika
Journal: Artif DNA PNA XNA Date: 2013 Apr-Jun

3. Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA.

Authors: Masayasu Kuwahara; Jun-ichi Nagashima; Masatoshi Hasegawa; Takehiro Tamura; Rina Kitagata; Kazuo Hanawa; Shin-ichi Hososhima; Toshiyuki Kasamatsu; Hiroaki Ozaki; Hiroaki Sawai
Journal: Nucleic Acids Res Date: 2006-09-29 Impact factor: 16.971

4. Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides.

Authors: Masayasu Kuwahara; Satoshi Obika; Jun-ichi Nagashima; Yuki Ohta; Yoshiyuki Suto; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi
Journal: Nucleic Acids Res Date: 2008-06-26 Impact factor: 16.971

Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR.

1. Substrate specificity and kinetic framework of a DNAzyme with an expanded chemical repertoire: a putative RNaseA mimic that catalyzes RNA hydrolysis independent of a divalent metal cation.

Review 2. In vitro selection of BNA (LNA) aptamers.

3. Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA.

4. Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides.

Review 5. Nucleic Acid aptamers: new methods for selection, stabilization, and application in biomedical science.

6. Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases.

7. Modified nucleoside triphosphates in bacterial research for in vitro and live-cell applications.

8. Artificial specific binders directly recovered from chemically modified nucleic acid libraries.

Review 9. Modified Nucleoside Triphosphates for In-vitro Selection Techniques.