| Literature DB >> 1455229 |
V Arondel1, B Lemieux, I Hwang, S Gibson, H M Goodman, C R Somerville.
Abstract
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.Entities:
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Year: 1992 PMID： 1455229 DOI： 10.1126/science.1455229
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728