Literature DB >> 14551896

Pathogen genotyping in polyclonal infections: application of a fluorogenic polymerase-chain-reaction assay in malaria.

Saskia Decuypere1, Ellen Elinck, Chantal Van Overmeir, Ambrose O Talisuna, Umberto D'Alessandro, Jean-Claude Dujardin.   

Abstract

Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1). how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2). how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples.

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Year:  2003        PMID： 14551896     DOI： 10.1086/378521

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  7 in total

1.  High-throughput genotyping of single nucleotide polymorphisms in the Plasmodium falciparum dhfr gene by asymmetric PCR and melt-curve analysis.

Authors:  Rochelle E Cruz; Sandra E Shokoples; Dammika P Manage; Stephanie K Yanow
Journal:  J Clin Microbiol       Date:  2010-07-14       Impact factor: 5.948

2.  Rapid real-time PCR genotyping of mutations associated with sulfadoxine-pyrimethamine resistance in Plasmodium falciparum.

Authors:  Alisa P Alker; Victor Mwapasa; Steven R Meshnick
Journal:  Antimicrob Agents Chemother       Date:  2004-08       Impact factor: 5.191

3.  Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum.

Authors:  Norbert P LeClair; Melissa D Conrad; Frederick N Baliraine; Christian Nsanzabana; Samuel L Nsobya; Philip J Rosenthal
Journal:  J Clin Microbiol       Date:  2013-05-29       Impact factor: 5.948

4.  Development of a TaqMan Allelic Discrimination assay for detection of single nucleotides polymorphisms associated with anti-malarial drug resistance.

Authors:  Edwin Kamau; Saba Alemayehu; Karla C Feghali; LaDonna S Tolbert; Bernard Ogutu; Christian F Ockenhouse
Journal:  Malar J       Date:  2012-01-20       Impact factor: 2.979

5.  Prevalence of Plasmodium falciparum field isolates with deletions in histidine-rich protein 2 and 3 genes in context with sub-Saharan Africa and India: a systematic review and meta-analysis.

Authors:  Loick P Kojom; Vineeta Singh
Journal:  Malar J       Date:  2020-01-28       Impact factor: 2.979

6.  Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum.

Authors:  Petra F Mens; Chantal van Overmeir; Maryline Bonnet; Jean-Claude Dujardin; Umberto d'Alessandro
Journal:  Malar J       Date:  2008-03-17       Impact factor: 2.979

7.  Validation of the ligase detection reaction fluorescent microsphere assay for the detection of Plasmodium falciparum resistance mediating polymorphisms in Uganda.

Authors:  Sheila Nankoberanyi; George W Mbogo; Norbert P LeClair; Melissa D Conrad; Patrick Tumwebaze; Stephen Tukwasibwe; Moses R Kamya; Jordan Tappero; Samuel L Nsobya; Philip J Rosenthal
Journal:  Malar J       Date:  2014-03-14       Impact factor: 2.979
  7 in total

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