Literature DB >> 14543704

Polymerase chain reaction (PCR) techniques for site-directed mutagenesis.

J Reikofski1, B Y Tao.   

Abstract

Polymerase chain reaction (PCR) technology has revolutionized the process of isolating and amplifying segments of DNA. One powerful application of PCR is its use in precise site-directed mutagenesis (SDM). SDM provides an elegant tool for scientists and engineers to explore biocatalytic mechanisms and processes to understand the structural-functional relationships of enzymes and other proteins. This article reviews techniques and methodology used in site-directed mutagenesis of genes by PCR.

Year:  1992        PMID： 14543704     DOI： 10.1016/0734-9750(92)91451-j

Source DB:  PubMed          Journal:  Biotechnol Adv        ISSN： 0734-9750            Impact factor:   14.227


  35 in total

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3.  Mutagenesis of Glu403 to Cys in rabbit neutral endopeptidase-24.11 (neprilysin) creates a disulphide-linked homodimer: analogy with endothelin-converting enzyme.

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Journal:  Biochem J       Date:  1997-11-01       Impact factor: 3.857

4.  Effect of site-specific mutations in different phosphotransfer domains of the chemosensory protein ChpA on Pseudomonas aeruginosa motility.

Authors:  Andrew J Leech; John S Mattick
Journal:  J Bacteriol       Date:  2006-09-29       Impact factor: 3.490

5.  Chemical modifications of a recombinant bovine stress-inducible 70 kDa heat-shock protein (Hsp70) mimics Hsp70 isoforms from tissues.

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7.  RecQL4 tethering on the pre-replicative complex induces unscheduled origin activation and replication stress in human cells.

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Journal:  J Biol Chem       Date:  2019-09-13       Impact factor: 5.157

8.  Cryptic protein interactions regulate DNA replication initiation.

Authors:  Lindsay A Matthews; Lyle A Simmons
Journal:  Mol Microbiol       Date:  2018-10-21       Impact factor: 3.501

9.  Rational design of a secreted enzymatically inactive mutant of extracellular superoxide dismutase.

Authors:  Adam J Case; James J Mezhir; Brianne R O'Leary; Jennifer E Hrabe; Frederick E Domann
Journal:  Redox Rep       Date:  2012       Impact factor: 4.412

10.  Allelic exchange and mutant selection demonstrate that common clinical embCAB gene mutations only modestly increase resistance to ethambutol in Mycobacterium tuberculosis.

Authors:  Hassan Safi; Robert D Fleischmann; Scott N Peterson; Marcus B Jones; Behnam Jarrahi; David Alland
Journal:  Antimicrob Agents Chemother       Date:  2009-10-12       Impact factor: 5.191
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