Literature DB >> 14532233

Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR.

Denis Filisetti1, Mohamed Gorcii, Elodie Pernot-Marino, Odile Villard, Ermanno Candolfi.   

Abstract

Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis.

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Year:  2003        PMID： 14532233      PMCID： PMC254317          DOI： 10.1128/JCM.41.10.4826-4828.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR.

Authors:  W L Homan; M Vercammen; J De Braekeleer; H Verschueren
Journal:  Int J Parasitol       Date:  2000-01       Impact factor: 3.981

2.  Contribution of a new PCR assay to the prenatal diagnosis of congenital toxoplasmosis.

Authors:  J Cazenave; F Forestier; M H Bessieres; B Broussin; J Begueret
Journal:  Prenat Diagn       Date:  1992-02       Impact factor: 3.050

3.  A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction.

Authors:  H Pelloux; J Weiss; J Simon; F Muet; H Fricker-Hidalgo; A Goullier-Fleuret; P Ambroise-Thomas
Journal:  FEMS Microbiol Lett       Date:  1996-04-15       Impact factor: 2.742

4.  Mother-to-child transmission of toxoplasmosis: risk estimates for clinical counselling.

Authors:  D Dunn; M Wallon; F Peyron; E Petersen; C Peckham; R Gilbert
Journal:  Lancet       Date:  1999-05-29       Impact factor: 79.321

5.  New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay.

Authors:  I Casas; L Powell; P E Klapper; G M Cleator
Journal:  J Virol Methods       Date:  1995-05       Impact factor: 2.014

6.  Strategy for diagnosis of congenital toxoplasmosis: evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and A antibodies.

Authors:  J M Pinon; H Dumon; C Chemla; J Franck; E Petersen; M Lebech; J Zufferey; M H Bessieres; P Marty; R Holliman; J Johnson; V Luyasu; B Lecolier; E Guy; D H Joynson; A Decoster; G Enders; H Pelloux; E Candolfi
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

7.  Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes.

Authors:  J M Costa; C Pautas; P Ernault; F Foulet; C Cordonnier; S Bretagne
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

Review 8.  Molecular diagnosis of toxoplasmosis.

Authors:  Patrick Bastien
Journal:  Trans R Soc Trop Med Hyg       Date:  2002-04       Impact factor: 2.184

9.  Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.

Authors:  J L Burg; C M Grover; P Pouletty; J C Boothroyd
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

10.  Detection of Toxoplasma gondii by PCR and tissue culture in cerebrospinal fluid and blood of human immunodeficiency virus-seropositive patients.

Authors:  M Dupon; J Cazenave; J L Pellegrin; J M Ragnaud; A Cheyrou; I Fischer; B Leng; J Y Lacut
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948
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  15 in total

Review 1.  Recent developments for diagnosis of toxoplasmosis.

Authors:  Jack S Remington; Philippe Thulliez; Jose G Montoya
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

2.  Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR.

Authors:  Wenli Yang; H D Alan Lindquist; Vitaliano Cama; Frank W Schaefer; Eric Villegas; Ronald Fayer; Earl J Lewis; Yaoyu Feng; Lihua Xiao
Journal:  Appl Environ Microbiol       Date:  2009-04-10       Impact factor: 4.792

3.  Serological prevalence of Toxoplasma gondii antibodies in pregnant women from Southern Brazil.

Authors:  Rogerio S Vaz; Vanete Thomaz-Soccol; Elaine Sumikawa; Ana Teresa B Guimarães
Journal:  Parasitol Res       Date:  2010-02       Impact factor: 2.289

4.  Optimization of Toxoplasma gondii DNA extraction from amniotic fluid using NucliSENS easyMAG and comparison with QIAamp DNA minikit.

Authors:  B Faucher; F Miermont; S Ranque; J Franck; R Piarroux
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-09-04       Impact factor: 3.267

Review 5.  Polymerase chain reaction in the diagnosis of congenital toxoplasmosis: more than two decades of development and evaluation.

Authors:  Rashad Abdul-Ghani
Journal:  Parasitol Res       Date:  2011-01-11       Impact factor: 2.289

6.  A 10-year retrospective comparison of two target sequences, REP-529 and B1, for Toxoplasma gondii detection by quantitative PCR.

Authors:  Sorya Belaz; Jean-Pierre Gangneux; Peggy Dupretz; Claude Guiguen; Florence Robert-Gangneux
Journal:  J Clin Microbiol       Date:  2015-02-04       Impact factor: 5.948

7.  Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis.

Authors:  Denis Filisetti; Hélène Yera; Odile Villard; Benoît Escande; Estelle Wafo; Véronique Houfflin-Debarge; Laurence Delhaes; Patrick Bastien
Journal:  J Clin Microbiol       Date:  2015-02-18       Impact factor: 5.948

8.  Comparison of methods for detection of Toxoplasma gondii in tissues of naturally exposed pigs.

Authors:  Rodrigo A Bezerra; Fábio S Carvalho; Luciana A Guimarães; Daniele S Rocha; Fabiana L Silva; Amauri A Wenceslau; George R Albuquerque
Journal:  Parasitol Res       Date:  2011-07-08       Impact factor: 2.289

9.  Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens.

Authors:  Yvon Sterkers; Emmanuelle Varlet-Marie; Sophie Cassaing; Marie-Pierre Brenier-Pinchart; Sophie Brun; Frédéric Dalle; Laurence Delhaes; Denis Filisetti; Hervé Pelloux; Hélène Yera; Patrick Bastien
Journal:  J Clin Microbiol       Date:  2010-07-07       Impact factor: 5.948

10.  Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid.

Authors:  H Yera; D Filisetti; P Bastien; T Ancelle; P Thulliez; L Delhaes
Journal:  J Clin Microbiol       Date:  2009-10-21       Impact factor: 5.948
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