Literature DB >> 14532225

Genetic diversity among clinical isolates of Candida glabrata analyzed by randomly amplified polymorphic DNA and multilocus enzyme electrophoresis analyses.

Xavier M Boldo1, Lourdes Villa-Tanaca, Gerardo Zúñiga, César Hernández-Rodríguez.   

Abstract

The genetic diversity of 47 clinical and reference strains of Candida glabrata from several geographical origins and diverse clinical disorders, with different antifungal susceptibilities, as well as their genetic relationships were studied through multilocus enzyme electrophoresis (MLEE) and randomly amplified polymorphic DNA (RAPD) techniques. The genetic diversity estimated for 11 MLEE loci measured as average heterozygosity (h) was 0.055. A high level of genetic relatedness among isolates was established by cluster analysis. Forty-nine RAPD markers were analyzed, and the average genetic diversity among isolates, estimated by Shannon's index (Ho), was 0.372. The PhiST values estimated through an analysis of molecular variance to assess genetic differentiation among isolates revealed no genetic differentiation among them. Our results revealed very low genetic diversity among isolates, a lack of differentiation, and no association with their geographic origin and the clinical characteristics.

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Year:  2003        PMID： 14532225      PMCID： PMC254345          DOI： 10.1128/JCM.41.10.4799-4804.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

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Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-05-21       Impact factor: 3.267

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Journal:  Eur J Pediatr       Date:  2009-12-04       Impact factor: 3.183

3.  Analyses of the genetic diversity and population structures of Histoplasma capsulatum clinical isolates from Mexico, Guatemala, Colombia and Argentina, using a randomly amplified polymorphic DNA-PCR assay.

Authors:  J H Sahaza; E Duarte-Escalante; C Canteros; G Rodríguez-Arellanes; M R Reyes-Montes; M L Taylor
Journal:  Epidemiol Infect       Date:  2019-01       Impact factor: 2.451

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