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Literature DB >> 14532217

Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene.

William Hurtle¹, Elizabeth Bode, Rebecca Susan Kaplan, Jeff Garrison, Brian Kearney, David Shoemaker, Erik Henchal, David Norwood.

Abstract

Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a method for identifying Bacillus anthracis by analyzing two chromosomal targets, the 16S-23S intergenic spacer region (ISR) and the gyrA gene. The 16S-23S ISR was analyzed by this method with 42 strains of B. anthracis, 36 strains of Bacillus cereus, and 12 strains of Bacillus thuringiensis; the gyrA gene was analyzed by this method with 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis. Two blind panels of 45 samples each were analyzed to evaluate the potential diagnostic capability of this method. Our results show that DHPLC is an efficient method for the identification of B. anthracis.

Entities: Chemical Species

Mesh：

Substances：

Year: 2003 PMID： 14532217 PMCID： PMC254356 DOI： 10.1128/JCM.41.10.4758-4766.2003

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

26 in total

Review 1. Anthrax.

Authors: T C Dixon; M Meselson; J Guillemin; P C Hanna
Journal: N Engl J Med Date: 1999-09-09 Impact factor: 91.245

2. Temperature-mediated heteroduplex analysis performed by using denaturing high-performance liquid chromatography to identify sequence polymorphisms in Mycobacterium tuberculosis complex organisms.

Authors: Robert C Cooksey; Glenn P Morlock; Brian P Holloway; Josef Limor; Michael Hepburn
Journal: J Clin Microbiol Date: 2002-05 Impact factor: 5.948

3. Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis.

Authors: T Kaneko; R Nozaki; K Aizawa
Journal: Microbiol Immunol Date: 1978 Impact factor: 1.955

4. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis--one species on the basis of genetic evidence.

Authors: E Helgason; O A Okstad; D A Caugant; H A Johansen; A Fouet; M Mock; I Hegna; A B Kolstø
Journal: Appl Environ Microbiol Date: 2000-06 Impact factor: 4.792

5. Molecular epidemiological analysis of the changing nature of a meningococcal outbreak following a vaccination campaign.

Authors: Liran I Shlush; Doron M Behar; Adrian Zelazny; Nathy Keller; James R Lupski; Arthur L Beaudet; Dani Bercovich
Journal: J Clin Microbiol Date: 2002-10 Impact factor: 5.948

6. Examination of single and multiple mutations involved in resistance to quinolones in Staphylococcus aureus by a combination of PCR and denaturing high-performance liquid chromatography (DHPLC).

Authors: Fatima Hannachi-M'Zali; Jane E Ambler; Claire F Taylor; Peter M Hawkey
Journal: J Antimicrob Chemother Date: 2002-11 Impact factor: 5.790

2. Pathogenic Acanthamoeba castellanii Secretes the Extracellular Aminopeptidase M20/M25/M40 Family Protein to Target Cells for Phagocytosis by Disruption.

Authors: Jian-Ming Huang; Chen-Chieh Liao; Chung-Ching Kuo; Lih-Ren Chen; Lynn L H Huang; Jyh-Wei Shin; Wei-Chen Lin
Journal: Molecules Date: 2017-12-18 Impact factor: 4.411

2 in total

Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene.

Review 1. Anthrax.

2. Temperature-mediated heteroduplex analysis performed by using denaturing high-performance liquid chromatography to identify sequence polymorphisms in Mycobacterium tuberculosis complex organisms.

3. Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis.

4. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis--one species on the basis of genetic evidence.

5. Molecular epidemiological analysis of the changing nature of a meningococcal outbreak following a vaccination campaign.

6. Examination of single and multiple mutations involved in resistance to quinolones in Staphylococcus aureus by a combination of PCR and denaturing high-performance liquid chromatography (DHPLC).

7. The DNA dependence of the ATPase activity of DNA gyrase.

8. Genetic relatedness within the Bacillus cereus group of bacilli.

9. Rapid and sensitive identification of pathogenic and apathogenic Bacillus anthracis by real-time PCR.

10. Denaturing HPLC for identifying bacteria.

1. Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe.

2. Pathogenic Acanthamoeba castellanii Secretes the Extracellular Aminopeptidase M20/M25/M40 Family Protein to Target Cells for Phagocytosis by Disruption.