PURPOSE: To clarify the effect of preincubation of oocytes on the results of IVF and ICSI. METHODS: A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm; fertilization rates, embryo quality, and pregnancy outcomes were analyzed in both IVF and ICSI arms according to the preincubation period duration of oocytes. RESULTS: The MII percentage of the ICSI arm was significantly lower (P < 0.05) in the group with preincubation period of < 2.5 h. The fertilization rates in groups with preincubation for 2.5-5.5 h were significantly higher (P < 0.001) for IVF. Embryo quality and pregnancy outcomes were not significantly different between the IVF or ICSI arm. CONCLUSIONS: The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.
PURPOSE: To clarify the effect of preincubation of oocytes on the results of IVF and ICSI. METHODS: A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm; fertilization rates, embryo quality, and pregnancy outcomes were analyzed in both IVF and ICSI arms according to the preincubation period duration of oocytes. RESULTS: The MII percentage of the ICSI arm was significantly lower (P < 0.05) in the group with preincubation period of < 2.5 h. The fertilization rates in groups with preincubation for 2.5-5.5 h were significantly higher (P < 0.001) for IVF. Embryo quality and pregnancy outcomes were not significantly different between the IVF or ICSI arm. CONCLUSIONS: The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.
Authors: L L Veeck; J W Wortham; J Witmyer; B A Sandow; A A Acosta; J E Garcia; G S Jones; H W Jones Journal: Fertil Steril Date: 1983-05 Impact factor: 7.329
Authors: Meghan B Smith; Jacqueline R Ho; Victoria Cortessis; Irene Jiyao Chen; Kristin A Bendikson; Richard J Paulson; Lynda K McGinnis; Ali Ahmady Journal: J Assist Reprod Genet Date: 2021-06-03 Impact factor: 3.357