[Studies on supercritical CO2 fluid extraction for ginkgolic acids in the epicarp of Ginkgo biloba].

The technology of supercritical CO2 fluid extraction for ginkgolic acids in the epicarp of Ginkgo biloba L. was studied. The effect of pressure, temperature and extraction time on the yield of the ginkgolic acids was explored. The optimum conditions for supercritical CO2 fluid extraction was 30 MPa, 45 degrees C, extraction time 6 h and the flow rate of CO2 2L/min. The ginkgolic acids were determined by HPLC. Supercritical CO2 fluid extraction exceled the traditional solvent extraction in high yield, high purification and easy operation.