BACKGROUND/ PURPOSE: Microgastria and postgastrectomy morbidities are substantial. The authors hypothesized a functional living tissue-engineered stomach could function as a replacement alternative. METHODS: Stomach organoid units, mesenchymal cores surrounded by epithelia, were isolated from neonatal and adult rats and transplanted paratopically on biodegradable polymer tubes, which were implanted in syngeneic hosts, varying the inclusion of stomach regions. Four weeks later, tissue-engineered stomach (TES) was either harvested or anastomosed. GFP labeling was performed before implantation. Histology and immunohistochemical detection of the antigensgastrin and actin smooth muscle were performed. RESULTS: Ninety-eight percent of all animals generated TES, including TES formation from adult tissue. Immunohistochemistry for alpha-actin smooth muscle and gastrin confirms the presence of a smooth muscle layer and a well-developed gastric epithelium containing all the elements of the native rat stomach including gastric pits and squamous layers, varying by included regions at harvest. TES architecture was maintained in anastomosis: GFP-labeled TES maintained signal in anastomosis, proving the donor origin of the TES. CONCLUSIONS: TES resembles native stomach and maintains robust histology in anastomosis, a new versatile model for the study of gastric physiology and possible therapy.
BACKGROUND/ PURPOSE: Microgastria and postgastrectomy morbidities are substantial. The authors hypothesized a functional living tissue-engineered stomach could function as a replacement alternative. METHODS: Stomach organoid units, mesenchymal cores surrounded by epithelia, were isolated from neonatal and adult rats and transplanted paratopically on biodegradable polymer tubes, which were implanted in syngeneic hosts, varying the inclusion of stomach regions. Four weeks later, tissue-engineered stomach (TES) was either harvested or anastomosed. GFP labeling was performed before implantation. Histology and immunohistochemical detection of the antigensgastrin and actin smooth muscle were performed. RESULTS: Ninety-eight percent of all animals generated TES, including TES formation from adult tissue. Immunohistochemistry for alpha-actin smooth muscle and gastrin confirms the presence of a smooth muscle layer and a well-developed gastric epithelium containing all the elements of the native rat stomach including gastric pits and squamous layers, varying by included regions at harvest. TES architecture was maintained in anastomosis: GFP-labeled TES maintained signal in anastomosis, proving the donor origin of the TES. CONCLUSIONS:TES resembles native stomach and maintains robust histology in anastomosis, a new versatile model for the study of gastric physiology and possible therapy.
Authors: Ryan Gregory Spurrier; Allison L Speer; Xiaogang Hou; Wael N El-Nachef; Tracy C Grikscheit Journal: Tissue Eng Part A Date: 2014-11-20 Impact factor: 3.845
Authors: Tracy C Grikscheit; Aleem Siddique; Erin R Ochoa; Ashok Srinivasan; Eben Alsberg; Richard A Hodin; Joseph P Vacanti Journal: Ann Surg Date: 2004-11 Impact factor: 12.969
Authors: Frédéric G Sala; Jamil A Matthews; Allison L Speer; Yasuhiro Torashima; Erik R Barthel; Tracy C Grikscheit Journal: Tissue Eng Part A Date: 2011-05-04 Impact factor: 3.845