| Literature DB >> 14520010 |
Rana Rustom1, Bohan Wang, Frank McArdle, Liliana Shalamanova, John Alexander, Anne McArdle, Carol E Thomas, J Michael Bone, Alan Shenkin, Malcolm J Jackson.
Abstract
Chronic metabolic acidosis occurs commonly in chronic renal failure (CRF). The proximal renal tubular cell is the site in the kidney of high oxidative metabolic activity and in CRF is associated with adaptive hypertrophy and hypermetabolism. We hypothesised that chronic acidosis may lead to increased generation of reactive oxygen species due to increased oxidative activity. We developed a novel model of chronic acidosis in LLC-PK1 cells and measured markers of oxidative stress and metabolism. Acidosis led to a reduction in cellular total glutathione and protein thiol content and an increase in glutathione peroxidase activity and NH3 generation. The expression of constitutively expressed heat stress protein (HSP) HSC70 and HSP60 increased at pH 7.0. Copyright 2003 S. Karger AG, BaselEntities:
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Year: 2003 PMID： 14520010 DOI： 10.1159/000073019
Source DB: PubMed Journal: Nephron Exp Nephrol ISSN： 1660-2129