| Literature DB >> 14515986 |
Hidehiko Shogomori1, Deborah A Brown.
Abstract
Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterol-rich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely-used method for studying lipid rafts takes advantage of their insolubility in non-ionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.Entities:
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Year: 2003 PMID： 14515986 DOI： 10.1515/BC.2003.139
Source DB: PubMed Journal: Biol Chem ISSN： 1431-6730 Impact factor: 3.915