Literature DB >> 14514050

Expression of the gene coding for bacterial hemoglobin improves beta-galactosidase production in a recombinant Pichia pastoris.

Jyh-Ming Wu1, Tsu-An Hsu, Cheng-Kang Lee.   

Abstract

Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidase activity to 4-fold higher than that of VHb-free cell in yeast extract/peptone/methanol medium under aerobic cultivation.

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Year:  2003        PMID： 14514050     DOI： 10.1023/a:1025072020791

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  4 in total

1.  Expression of Bacillus licheniformis α-amylase in Pichia pastoris without antibiotics-resistant gene and effects of glycosylation on the enzymic thermostability.

Authors:  Xinlin Hu; Xin Yuan; Nisha He; Tony Z Zhuang; Pan Wu; Guimin Zhang
Journal:  3 Biotech       Date:  2019-10-29       Impact factor: 2.406

2.  Chimeric antibody-binding Vitreoscilla hemoglobin (VHb) mediates redox-catalysis reaction: new insight into the functional role of VHb.

Authors:  Yaneenart Suwanwong; Malin Kvist; Chartchalerm Isarankura-Na-Ayudhya; Natta Tansila; Leif Bulow; Virapong Prachayasittikul
Journal:  Int J Biol Sci       Date:  2006-08-22       Impact factor: 6.580

3.  Regulation of methanol utilisation pathway genes in yeasts.

Authors:  Franz S Hartner; Anton Glieder
Journal:  Microb Cell Fact       Date:  2006-12-14       Impact factor: 5.328

Review 4.  Vitreoscilla Haemoglobin: A Tool to Reduce Overflow Metabolism.

Authors:  Hilal Taymaz-Nikerel; Alvaro R Lara
Journal:  Microorganisms       Date:  2021-12-26
  4 in total

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