Literature DB >> 14510508

Cloning and expression of bglF gene from alkaliphilic Nocardiopsis sp. strain F96.

Sumiko Masuda1, Kimiko Endo, Tokusuke Hayami, Tetsuya Fukazawa, Rie Yatsunami, Satoshi Nakamura.   

Abstract

The gene encoding a novel beta-1,3-glucanase was cloned from alkaliphilic Nocardiopsis sp. F96 and sequenced. The gene contained an open reading frame of 936 bp. The deduced amino acid sequence of the beta-1,3-glucanase exhibited highest homology to those of family 16 glucanases, suggesting that the enzyme belonged to family 16. The beta-1,3-glucanase gene was functionally expressed in Escherichia coli.

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Year:  2003        PMID: 14510508     DOI: 10.1093/nass/3.1.317

Source DB:  PubMed          Journal:  Nucleic Acids Res Suppl


  1 in total

1.  Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from alkaliphilic Nocardiopsis sp. strain F96.

Authors:  Guntur Fibriansah; Sumiko Masuda; Raita Hirose; Kensaku Hamada; Nobuo Tanaka; Satoshi Nakamura; Takashi Kumasaka
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-12-16
  1 in total

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