BACKGROUND: RBC volume, 24-hour posttransfusion survival, and life span can be measured with radio-isotopes and nonradioactive procedures. STUDY DESIGN AND METHODS: RBC volume was measured directly with autologous baboon RBCs labeled with biotin-X-N-hydroxysuccinimide (NHS), 51Cr, 99mTc, and 111In-oxine and indirectly from the 125I plasma volume and the total body Hct. Twenty-four-hour posttransfusion survival and life span were measured in autologous fresh baboon RBCs labeled with 51Cr, 111In-oxine, 99mTc, and biotin-X-NHS. RESULTS: Significantly larger RBC volumes were observed when the fresh autologous RBCs were labeled with 51Cr, 111In-oxine, or 99mTc than when they were labeled with the nonradioactive biotin-X-NHS. Twenty-four-hour posttransfusion survival values were significantly lower in the RBCs labeled with 111In-oxine or 99mTc than in the RBCs labeled with 51Cr. CONCLUSIONS: The greater in vivo elution of 51Cr, 111In-oxine, and 99mTc than that of biotin-X-NHS influenced the measurements of RBC volume, 24-hour posttransfusion survival, and life span of the fresh baboon RBCs.
BACKGROUND: RBC volume, 24-hour posttransfusion survival, and life span can be measured with radio-isotopes and nonradioactive procedures. STUDY DESIGN AND METHODS: RBC volume was measured directly with autologous baboon RBCs labeled with biotin-X-N-hydroxysuccinimide (NHS), 51Cr, 99mTc, and 111In-oxine and indirectly from the 125I plasma volume and the total body Hct. Twenty-four-hour posttransfusion survival and life span were measured in autologous fresh baboon RBCs labeled with 51Cr, 111In-oxine, 99mTc, and biotin-X-NHS. RESULTS: Significantly larger RBC volumes were observed when the fresh autologous RBCs were labeled with 51Cr, 111In-oxine, or 99mTc than when they were labeled with the nonradioactive biotin-X-NHS. Twenty-four-hour posttransfusion survival values were significantly lower in the RBCs labeled with 111In-oxine or 99mTc than in the RBCs labeled with 51Cr. CONCLUSIONS: The greater in vivo elution of 51Cr, 111In-oxine, and 99mTc than that of biotin-X-NHS influenced the measurements of RBC volume, 24-hour posttransfusion survival, and life span of the fresh baboon RBCs.
Authors: Donald M Mock; Nell I Matthews; Shan Zhu; Leon F Burmeister; M Bridget Zimmerman; Ronald G Strauss; Robert L Schmidt; Demet Nalbant; Kevin J Freise; Peter Veng-Pedersen; John A Widness Journal: Transfusion Date: 2010-12 Impact factor: 3.157
Authors: Donald M Mock; Nell I Matthews; Shan Zhu; Leon F Burmeister; M Bridget Zimmerman; Ronald G Strauss; Robert L Schmidt; Demet Nalbant; Gretchen A Cress; John A Widness Journal: Transfusion Date: 2011-01 Impact factor: 3.157
Authors: Donald M Mock; Nell I Matthews; Ronald G Strauss; Leon F Burmeister; Robert Schmidt; John A Widness Journal: Transfusion Date: 2009-02-10 Impact factor: 3.157
Authors: Alberto Moreno; Monica Cabrera-Mora; Anapatricia Garcia; Jack Orkin; Elizabeth Strobert; John W Barnwell; Mary R Galinski Journal: Infect Immun Date: 2013-03-18 Impact factor: 3.441
Authors: Ronald G Strauss; Donald M Mock; John A Widness; Karen Johnson; Gretchen Cress; Robert L Schmidt Journal: Transfusion Date: 2004-06 Impact factor: 3.157