OBJECTIVE: To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA). DESIGN: Controlled experimental study. SETTING: The animal laboratory of the Experimental Surgery Center of Ege University, Izmir, Turkey. ANIMAL(S): Ten nondiabetic (control) and 20 diabetic adult male albino rats. INTERVENTION(S): The rats were IP injected with 40 mg/kg streptozotocin for 5 days to create a diabetic condition that was confirmed by testing blood glucose levels 5-7 days after the first injection. MAIN OUTCOME MEASURE(S): Mean testicular diameter, mean semniferous tubuli diameter (MSTD), and PCNA index. RESULT(S): Six weeks after the streptozotocin injection, both the mean testicular and the seminiferous tubuli diameters were significantly decreased in diabetic rats compared with the control group. CONCLUSION(S): The effects of diabetes on spermatogenesis can be clearly detected as a decrease in testicular diameter, MSTD, and PCNA index.
OBJECTIVE: To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA). DESIGN: Controlled experimental study. SETTING: The animal laboratory of the Experimental Surgery Center of Ege University, Izmir, Turkey. ANIMAL(S): Ten nondiabetic (control) and 20 diabetic adult male albino rats. INTERVENTION(S): The rats were IP injected with 40 mg/kg streptozotocin for 5 days to create a diabetic condition that was confirmed by testing blood glucose levels 5-7 days after the first injection. MAIN OUTCOME MEASURE(S): Mean testicular diameter, mean semniferous tubuli diameter (MSTD), and PCNA index. RESULT(S): Six weeks after the streptozotocin injection, both the mean testicular and the seminiferous tubuli diameters were significantly decreased in diabeticrats compared with the control group. CONCLUSION(S): The effects of diabetes on spermatogenesis can be clearly detected as a decrease in testicular diameter, MSTD, and PCNA index.
Authors: Toyin Dorcas Alabi; Charon de Villiers; Stephan S du Plessis; Thomas K Monsees; Nicole L Brooks; Oluwafemi Omoniyi Oguntibeju Journal: Diabetes Metab Syndr Obes Date: 2020-11-23 Impact factor: 3.168
Authors: Glaura S A Fernandes; Carla D B Fernandez; Kleber E Campos; Débora C Damasceno; Janete A Anselmo-Franci; Wilma D G Kempinas Journal: Reprod Biol Endocrinol Date: 2011-07-27 Impact factor: 5.211
Authors: Breno H Caneguim; Paulo S Cerri; Luís C Spolidório; Sandra M Miraglia; Estela Sasso-Cerri Journal: Reprod Biol Endocrinol Date: 2009-02-25 Impact factor: 5.211