OBJECTIVE: To evaluate the effects of leptin on the production of interleukin (IL)-6 family cytokines and chemokines by human endometrial stromal cells (ESC) and epithelial cells. DESIGN: The effects of leptin on the production of IL-6, IL-11, leukemia inhibitory factor (LIF), IL-8, growth-regulated oncogene (GRO)-alpha, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-3alpha by ESC and the endometrial epithelial cell line HHUA were investigated. SETTING: Research laboratory at a medical university. PATIENT(S): Eight endometrial specimens in the late proliferative phase were used for the isolation of ESC. INTERVENTION(S): ESC and HHUA were incubated for 24 hours with recombinant human leptin. MAIN OUTCOME MEASURE(S): The concentration of IL-6, IL-11, LIF, IL-8, GRO-alpha, MCP-1, and MIP-3alpha were measured using ELISAs. RESULT(S): Unstimulated ESC and HHUA constitutively secreted IL-6, IL-11, LIF, IL-8, GRO-alpha, MCP-1, and MIP-3alpha. The increase in levels of IL-6, IL-8, GRO-alpha, MCP-1, and MIP-3alpha in the culture media of ESC and HHUA paralleled the addition of increasing amounts of leptin. In contrast, the levels of IL-11 and LIF were not affected by leptin administration. CONCLUSION(S): The present findings suggest that leptin may be an additional modulator of IL-6 and chemokine expression in the endometrium. Leptin may contribute to the normal and pathological processes of human reproduction by the regulation of these cytokines in the local environment.
OBJECTIVE: To evaluate the effects of leptin on the production of interleukin (IL)-6 family cytokines and chemokines by human endometrial stromal cells (ESC) and epithelial cells. DESIGN: The effects of leptin on the production of IL-6, IL-11, leukemia inhibitory factor (LIF), IL-8, growth-regulated oncogene (GRO)-alpha, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-3alpha by ESC and the endometrial epithelial cell line HHUA were investigated. SETTING: Research laboratory at a medical university. PATIENT(S): Eight endometrial specimens in the late proliferative phase were used for the isolation of ESC. INTERVENTION(S): ESC and HHUA were incubated for 24 hours with recombinant humanleptin. MAIN OUTCOME MEASURE(S): The concentration of IL-6, IL-11, LIF, IL-8, GRO-alpha, MCP-1, and MIP-3alpha were measured using ELISAs. RESULT(S): Unstimulated ESC and HHUA constitutively secreted IL-6, IL-11, LIF, IL-8, GRO-alpha, MCP-1, and MIP-3alpha. The increase in levels of IL-6, IL-8, GRO-alpha, MCP-1, and MIP-3alpha in the culture media of ESC and HHUA paralleled the addition of increasing amounts of leptin. In contrast, the levels of IL-11 and LIF were not affected by leptin administration. CONCLUSION(S): The present findings suggest that leptin may be an additional modulator of IL-6 and chemokine expression in the endometrium. Leptin may contribute to the normal and pathological processes of human reproduction by the regulation of these cytokines in the local environment.
Authors: Cecilia Carino; Alexander B Olawaiye; Salandre Cherfils; Takehiro Serikawa; Maureen P Lynch; Bo R Rueda; Ruben R Gonzalez Journal: Int J Cancer Date: 2008-12-15 Impact factor: 7.396
Authors: Yuan You; Patrick Stelzl; Dana N Joseph; Paulomi B Aldo; Anthony J Maxwell; Nava Dekel; Aihua Liao; Shannon Whirledge; Gil Mor Journal: Front Immunol Date: 2021-11-01 Impact factor: 7.561