OBJECTIVE: To determine whether the peripheral blood concentration of CD56(+)/CD16(-)/CD3(-) cells, the main natural killer (NK) subpopulation that colonizes the endometrium in the middle and late secretory phase, can be related to fertility or infertility status. DESIGN: A case control study. SETTING: Immunopathology department of an infertility laboratory. PATIENTS: A total 99 women were selected (group I: consecutive spontaneous aborters, n = 25; group II: sporadic spontaneous aborters, n = 30; group III: infertile, n = 33; group IV: controls, n = 11). INTERVENTION: Immunophenotyping of women grouped according to their fertility status. MAIN OUTCOME MEASURES: Peripheral blood lymphocytes were examined by two- and three-color flow cytometry. RESULTS: A statistically significant association between endometrial-type peripheral blood (PB) NK cell concentrations and fertility status (groups I and IV vs. groups II and III) was documented. The %(/TOTAL PB)(CD56+CD16-CD3-) NK cells was significantly higher [1] in fertile (groups I and IV) than in sporadic aborters/infertile (groups II and III) women, [2] in group I when compared with groups II and III, and [3] in group IV when compared with groups II and III. CONCLUSIONS: This study indicates that diminished numbers of the %(/TOTAL PB)(CD56+CD16-CD3-) NK cells are related to sporadic aborters and infertile women. Thus, the fraction could be used as an indicator of subsequent successful implantation and maintenance of gestation.
OBJECTIVE: To determine whether the peripheral blood concentration of CD56(+)/CD16(-)/CD3(-) cells, the main natural killer (NK) subpopulation that colonizes the endometrium in the middle and late secretory phase, can be related to fertility or infertility status. DESIGN: A case control study. SETTING: Immunopathology department of an infertility laboratory. PATIENTS: A total 99 women were selected (group I: consecutive spontaneous aborters, n = 25; group II: sporadic spontaneous aborters, n = 30; group III: infertile, n = 33; group IV: controls, n = 11). INTERVENTION: Immunophenotyping of women grouped according to their fertility status. MAIN OUTCOME MEASURES: Peripheral blood lymphocytes were examined by two- and three-color flow cytometry. RESULTS: A statistically significant association between endometrial-type peripheral blood (PB) NK cell concentrations and fertility status (groups I and IV vs. groups II and III) was documented. The %(/TOTAL PB)(CD56+CD16-CD3-) NK cells was significantly higher [1] in fertile (groups I and IV) than in sporadic aborters/infertile (groups II and III) women, [2] in group I when compared with groups II and III, and [3] in group IV when compared with groups II and III. CONCLUSIONS: This study indicates that diminished numbers of the %(/TOTAL PB)(CD56+CD16-CD3-) NK cells are related to sporadic aborters and infertile women. Thus, the fraction could be used as an indicator of subsequent successful implantation and maintenance of gestation.
Authors: Elias K Halvas; Kevin W Joseph; Leah D Brandt; Shuang Guo; Michele D Sobolewski; Jana L Jacobs; Camille Tumiotto; John K Bui; Joshua C Cyktor; Brandon F Keele; Gene D Morse; Michael J Bale; Wei Shao; Mary F Kearney; John M Coffin; Jason W Rausch; Xiaolin Wu; Stephen H Hughes; John W Mellors Journal: J Clin Invest Date: 2020-11-02 Impact factor: 14.808
Authors: Sarah E Dickinson; Brock A Griffin; Michelle F Elmore; Lisa Kriese-Anderson; Joshua B Elmore; Paul W Dyce; Soren P Rodning; Fernando H Biase Journal: BMC Genomics Date: 2018-02-09 Impact factor: 3.969
Authors: M Králícková; Z Ulcová-Gallová; R Síma; T Vanecek; P Síma; J Krizan; R Suchá; P Uher; O Hes; Z Novotný; Z Rokyta; V Vetvicka Journal: Folia Microbiol (Praha) Date: 2007 Impact factor: 2.629