| Literature DB >> 14503854 |
Jin Tae Jeon1, Eung Woo Park, Hyo Jeong Jeon, Tae Hun Kim, Kyung Tai Lee, Il Cheong Cheong.
Abstract
A porcine genomic bacterial artificial chromosome (BAC) library was constructed by cloning partial EcoRI-digested high-molecular-weight DNA from a Korean native boar into the EcoRI site of the pBACe3.6 vector. The library consists of about 165,000 clones with an average insert size of 125 kb, representing about seven genome equivalents of coverage. About 130,000 clones (corresponding to fivefold genome coverage) were arrayed in 14 superpools which were organized as four dimensional pools. The library was further characterized by PCR screening of 38 microsatellite probes. An average of 4.84 positive clones were selected per marker. This indicates that the library is unbiased and will be useful for initiating fine scale physical mapping of major QTL in pigs. The library is being used to isolate specific clones by screening with type I and type II marker clones located in the QTL region affecting intramuscular fat content on SSC6.Entities:
Mesh：
Year: 2003 PMID： 14503854
Source DB: PubMed Journal: Mol Cells ISSN： 1016-8478 Impact factor: 5.034