| Literature DB >> 14502277 |
Giovanni Di Pasquale1, Beverly L Davidson, Colleen S Stein, Inês Martins, Dominic Scudiero, Anne Monks, John A Chiorini.
Abstract
Understanding the process of vector transduction has important implications for the application and optimal use of a vector system for human gene therapy. Recent studies with vectors based on adeno-associated virus type 5 (AAV-5) have shown utility of this vector system in the lung, central nervous system, muscle and eye. To understand the natural tropism of this virus and to identify proteins necessary for AAV-5 transduction, we characterized 43 cell lines as permissive or nonpermissive for AAV-5 transduction and compared the gene expression profiles derived from cDNA microarray analyses of those cell lines. A statistically significant correlation was observed between expression of the platelet-derived growth factor receptor (PDGFR-alpha-polypeptide) and AAV-5 transduction. Subsequent experiments confirmed the role of PDGFR-alpha and PDGFR-beta as receptors for AAV-5. The tropism of AAV-5 in vivo also correlated with the expression pattern of PDGFR-alpha.Entities:
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Year: 2003 PMID： 14502277 DOI： 10.1038/nm929
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440