Literature DB >> 14501036

Purification of G protein subunits from Sf9 insect cells using hexahistidine-tagged alpha and beta gamma subunits.

Tohru Kozasa1.   

Abstract

G protein-mediated pathways are the most fundamental mechanisms of cell signaling. In order to analyze these pathways, the availability of purified recombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.

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Year:  2004        PMID： 14501036     DOI： 10.1385/1-59259-430-1:21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  22 in total

1.  Structural basis for the specific inhibition of heterotrimeric Gq protein by a small molecule.

Authors:  Akiyuki Nishimura; Ken Kitano; Jun Takasaki; Masatoshi Taniguchi; Norikazu Mizuno; Kenji Tago; Toshio Hakoshima; Hiroshi Itoh
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-16       Impact factor: 11.205

2.  A new approach to producing functional G alpha subunits yields the activated and deactivated structures of G alpha(12/13) proteins.

Authors:  Barry Kreutz; Douglas M Yau; Mark R Nance; Shihori Tanabe; John J G Tesmer; Tohru Kozasa
Journal:  Biochemistry       Date:  2006-01-10       Impact factor: 3.162

3.  Heterodimerization with Its splice variant blocks the ghrelin receptor 1a in a non-signaling conformation: a study with a purified heterodimer assembled into lipid discs.

Authors:  Sophie Mary; Jean-Alain Fehrentz; Marjorie Damian; Gérald Gaibelet; Hélène Orcel; Pascal Verdié; Bernard Mouillac; Jean Martinez; Jacky Marie; Jean-Louis Banères
Journal:  J Biol Chem       Date:  2013-07-09       Impact factor: 5.157

4.  The complex of G protein regulator RGS9-2 and Gβ(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum.

Authors:  Keqiang Xie; Ikuo Masuho; Cameron Brand; Carmen W Dessauer; Kirill A Martemyanov
Journal:  Sci Signal       Date:  2012-08-28       Impact factor: 8.192

5.  The pleckstrin homology domain of phospholipase Cbeta transmits enzymatic activation through modulation of the membrane-domain orientation.

Authors:  Guillaume Drin; Dominique Douguet; Suzanne Scarlata
Journal:  Biochemistry       Date:  2006-05-09       Impact factor: 3.162

6.  Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag.

Authors:  Baley A Fong; Alison R Gillies; Iraj Ghazi; Gary LeRoy; Kathleen C Lee; Lars F Westblade; David W Wood
Journal:  Protein Sci       Date:  2010-06       Impact factor: 6.725

7.  PI(3,4,5)P3 potentiates phospholipase C-beta activity.

Authors:  Yong Zhang; Sun Hyung Kwon; Walter K Vogel; Theresa M Filtz
Journal:  J Recept Signal Transduct Res       Date:  2009       Impact factor: 2.092

8.  Origin of the voltage dependence of G-protein regulation of P/Q-type Ca2+ channels.

Authors:  Yun Zhang; Yu-Hang Chen; Saroja D Bangaru; Linling He; Kathryn Abele; Shihori Tanabe; Tohru Kozasa; Jian Yang
Journal:  J Neurosci       Date:  2008-12-24       Impact factor: 6.167

9.  Quantitative Multiple-Reaction Monitoring Proteomic Analysis of Gβ and Gγ Subunits in C57Bl6/J Brain Synaptosomes.

Authors:  Yun Young Yim; W Hayes McDonald; Karren Hyde; Osvaldo Cruz-Rodríguez; John J G Tesmer; Heidi E Hamm
Journal:  Biochemistry       Date:  2017-09-21       Impact factor: 3.162

10.  GHSR-D2R heteromerization modulates dopamine signaling through an effect on G protein conformation.

Authors:  Marjorie Damian; Véronique Pons; Pedro Renault; Céline M'Kadmi; Bartholomé Delort; Lucie Hartmann; Ali I Kaya; Maxime Louet; Didier Gagne; Khoubaib Ben Haj Salah; Séverine Denoyelle; Gilles Ferry; Jean A Boutin; Renaud Wagner; Jean-Alain Fehrentz; Jean Martinez; Jacky Marie; Nicolas Floquet; Céline Galès; Sophie Mary; Heidi E Hamm; Jean-Louis Banères
Journal:  Proc Natl Acad Sci U S A       Date:  2018-04-09       Impact factor: 11.205
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