OBJECTIVE: A diabetic mouse model of accelerated neointimal formation would be a useful tool to understand the increased incidence of restenosis in patients with diabetes. METHODS AND RESULTS: Femoral artery endoluminal wire injury was performed in diabetic insulin 2 Akita (ins2Akita) and leptin receptor db/db (leprdb/db) mutant mice. Neointima size in ins2Akita mouse arteries was unchanged compared with nondiabetic wild-type littermates. Although Ki67 labeling demonstrated similar rates of replication in the neointima of leprdb/db mouse arteries, neointimal formation in leprdb/db mice was surprisingly reduced by approximately 90% compared with nondiabetic lepr+/+ mice. Four hours after arterial injury, medial smooth muscle cell death was diminished in leprdb/db arteries, suggesting that the initial response to arterial injury was altered in leprdb/db mice. CONCLUSIONS: These studies highlight a differential response to arterial injury in leprdb/db mice and suggest a potential role for leptin in the regulation of neointimal formation in response to arterial injury.
OBJECTIVE: A diabeticmouse model of accelerated neointimal formation would be a useful tool to understand the increased incidence of restenosis in patients with diabetes. METHODS AND RESULTS:Femoral artery endoluminal wire injury was performed in diabetic insulin 2 Akita (ins2Akita) and leptin receptor db/db (leprdb/db) mutant mice. Neointima size in ins2Akita mouse arteries was unchanged compared with nondiabetic wild-type littermates. Although Ki67 labeling demonstrated similar rates of replication in the neointima of leprdb/db mouse arteries, neointimal formation in leprdb/db mice was surprisingly reduced by approximately 90% compared with nondiabetic lepr+/+ mice. Four hours after arterial injury, medial smooth muscle cell death was diminished in leprdb/db arteries, suggesting that the initial response to arterial injury was altered in leprdb/db mice. CONCLUSIONS: These studies highlight a differential response to arterial injury in leprdb/db mice and suggest a potential role for leptin in the regulation of neointimal formation in response to arterial injury.
Authors: Suvi T Ruohonen; Ken Abe; Mia Kero; Laura Toukola; Saku Ruohonen; Matias Röyttä; Markku Koulu; Ullamari Pesonen; Zofia Zukowska; Eriika Savontaus Journal: Peptides Date: 2008-12-24 Impact factor: 3.750
Authors: Marco R Schroeter; Jacob Schneiderman; Bettina Schumann; Roland Glückermann; Petros Grimmas; Arnd B Buchwald; Theodor Tirilomis; Friedrich A Schöndube; Stavros V Konstantinides; Katrin Schäfer Journal: Histochem Cell Biol Date: 2007-08-07 Impact factor: 4.304
Authors: Jian Shan; Thomas B Nguyen; Hana Totary-Jain; Hayes Dansky; Steven O Marx; Andrew R Marks Journal: Proc Natl Acad Sci U S A Date: 2008-11-19 Impact factor: 11.205