Literature DB >> 14500026

A microplate fluorimetric assay for measuring dehalogenase activity.

Julian R Marchesi1.   

Abstract

A fluorimetric assay was developed to measure halide release from halogenated compounds being degraded by microbes. The method relies on the property of halides to quench the fluorescence of 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) by collision quenching. The assay shows a wide response to halide concentration (1-500 mM) and tolerates a wide pH range. Furthermore, it is simple to use, has the potential for automation and uses an inexpensive non-toxic reagent.

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Year:  2003        PMID: 14500026     DOI: 10.1016/s0167-7012(03)00132-5

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  2 in total

1.  An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation.

Authors:  Aşkın S Aslan-Üzel; Andy Beier; David Kovář; Clemens Cziegler; Santosh K Padhi; Eva D Schuiten; Mark Dörr; Dominique Böttcher; Frank Hollmann; Florian Rudroff; Marko D Mihovilovic; Tomáš Buryška; Jiří Damborský; Zbyněk Prokop; Christoffel P S Badenhorst; Uwe T Bornscheuer
Journal:  ChemCatChem       Date:  2020-01-31       Impact factor: 5.686

2.  A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay.

Authors:  Sebastian Fabritz; Franziska Maaß; Olga Avrutina; Tim Heiseler; Björn Steinmann; Harald Kolmar
Journal:  AMB Express       Date:  2012-09-24       Impact factor: 3.298

  2 in total

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