Literature DB >> 14499906

Thermodynamics of denaturation of complexes of barnase and binase with barstar.

Vladimir A Mitkevich1, Alexey A Schulga, Yaroslav S Ermolyuk, Vladimir M Lobachov, Vladimir O Chekhov, Gennady I Yakovlev, Robert W Hartley, C Nick Pace, Mikhail P Kirpichnikov, Alexander A Makarov.   

Abstract

Differential scanning calorimetry was used to study the thermodynamics of denaturation of protein complexes for which the free energy stabilizing the complexes varied between -8 and -16 kcal/mol. The proteins studied were the ribonucleases barnase and binase, their inhibitor barstar and mutants thereof, and complexes between the two. The results are in good agreement with the model developed by Brandts and Lin for studying the thermodynamics of denaturation for tight complexes between two proteins which undergo two-state thermal unfolding transitions.

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Year:  2003        PMID： 14499906     DOI： 10.1016/s0301-4622(03)00103-0

Source DB:  PubMed          Journal:  Biophys Chem        ISSN： 0301-4622            Impact factor:   2.352


  6 in total

1.  Free energies of protein-protein association determined by electrospray ionization mass spectrometry correlate accurately with values obtained by solution methods.

Authors:  Sanjay R Krishnaswamy; Evan R Williams; Jack F Kirsch
Journal:  Protein Sci       Date:  2006-06       Impact factor: 6.725

2.  The molecular chaperone Hsp70 from the thermotolerant Diptera species differs from the Drosophila paralog in its thermostability and higher refolding capacity at extreme temperatures.

Authors:  David G Garbuz; Dmitry Sverchinsky; Artem Davletshin; Boris A Margulis; Vladimir Mitkevich; Aleksei M Kulikov; Michael B Evgen'ev
Journal:  Cell Stress Chaperones       Date:  2019-10-30       Impact factor: 3.667

3.  CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex.

Authors:  Yury Kostyukevich; Aleksej A Shulga; Alexey Kononikhin; Igor Popov; Eugene Nikolaev; Sergey Deyev
Journal:  Sci Rep       Date:  2017-07-21       Impact factor: 4.379

Review 4.  Current Experimental Methods for Characterizing Protein-Protein Interactions.

Authors:  Mi Zhou; Qing Li; Renxiao Wang
Journal:  ChemMedChem       Date:  2016-02-11       Impact factor: 3.466

5.  Role of κ→λ light-chain constant-domain switch in the structure and functionality of A17 reactibody.

Authors:  Natalia Ponomarenko; Spyros D Chatziefthimiou; Inna Kurkova; Yuliana Mokrushina; Yuliana Mokrushina; Anastasiya Stepanova; Ivan Smirnov; Marat Avakyan; Tatyana Bobik; Azad Mamedov; Vladimir Mitkevich; Alexey Belogurov; Olga S Fedorova; Michael Dubina; Andrey Golovin; Victor Lamzin; Alain Friboulet; Alexander A Makarov; Matthias Wilmanns; Alexander Gabibov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2014-02-15

6.  Molten globule-like transition state of protein barnase measured with calorimetric force spectroscopy.

Authors:  Marc Rico-Pasto; Annamaria Zaltron; Sebastian J Davis; Silvia Frutos; Felix Ritort
Journal:  Proc Natl Acad Sci U S A       Date:  2022-03-10       Impact factor: 12.779
  6 in total

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