Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.

Literature DB >> 14491776

Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.

Abstract

A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H(3) into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.

Entities: Chemical

Keywords: NUCLEOSIDES AND NUCLEOTIDES/metabolism; PLANTS/metabolism; RIBONUCLEIC ACID/metabolism

Mesh：

Substances：

Year: 1962 PMID： 14491776 PMCID： PMC2106106 DOI： 10.1083/jcb.14.2.183

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

17 in total

1. Nucleotide composition of various ribosomes.

Authors: J M WALLACE; P O TS'O
Journal: Biochem Biophys Res Commun Date: 1961-06-02 Impact factor: 3.575

2. A survey of metabolic studies on isolated mammalian nuclei.

Authors: D B ROODYN
Journal: Int Rev Cytol Date: 1959

3. Synthesis of ribonucleic acid in plants. I. Distribution of ribonucleic acid and of protein among subcellular components of pea epicotyls.

Authors: P O TS'O; C S SATO
Journal: Exp Cell Res Date: 1959-05 Impact factor: 3.905

Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.

1. Nucleotide composition of various ribosomes.

2. A survey of metabolic studies on isolated mammalian nuclei.

3. Synthesis of ribonucleic acid in plants. I. Distribution of ribonucleic acid and of protein among subcellular components of pea epicotyls.

4. The choice of the suspension medium for rat-liver-cell nuclei.

5. Studies on isolated cell components. VI. The effects of nucleases and proteases on rat liver nuclei.

6. THE INCORPORATION OF LEUCINE-C INTO PROTEIN BY A CELL-FREE PREPARATION FROM MAIZE KERNELS.

7. THE ROLE OF DEOXYRIBONUCLEIC ACID AND OTHER POLYNUCLEOTIDES IN ATP SYNTHESIS BY ISOLATED CELL NUCLEI.

8. A Simple Tissue Homogenizer.

9. Determination of serum proteins by means of the biuret reaction.

10. The isolation of wheat germ nuclei and some aspects of their glycolytic metabolism.

1. ON THE NATURE OF NUCLEOLAR RNA.

2. SYNTHESIS OF TRANSFER RNA BY ISOLATED NUCLEI.

3. Isolation of nuclei from rice tissue grown in suspension culture.

4. [Taxonomical study of the genus Leucanthemum using cytophotometry].

5. Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.

6. Chromatin- and nuclei-directed ribonucleic Acid synthesis in sugar beet root.

7. Protein synthesis by isolated pea nucleoli.

8. ISOLATION OF THE GOLGI APPARATUS FROM PLANT CELLS.

9. Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue.