Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide.

Literature DB >> 14483299

Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide.

S L PALAY, S M McGEE-RUSSELL, S GORDON, M A GRILLO.

Abstract

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.

Entities: Chemical

Keywords: HISTOLOGICAL TECHNIQUES; MICROSCOPY, ELECTRON; NEURONS/anatomy and histology; OSMIUM

Mesh：

Substances：

Year: 1962 PMID： 14483299 PMCID： PMC2106034 DOI： 10.1083/jcb.12.2.385

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

20 in total

1. Phase and electron microscopic studies of experimental demyelination. I. Variations in myelin sheath contour in normal guinea pig sciatic nerve.

Authors: H D WEBSTER; D SPIRO
Journal: J Neuropathol Exp Neurol Date: 1960-01 Impact factor: 3.685

2. The fine structure of secretory neurons in the preoptic nucleus of the goldish (Carassius auratus).

Authors: S L PALAY
Journal: Anat Rec Date: 1960-12

3. [Electron microscopic observations of the neurohypophysis].

Authors: W BARGMANN; A KNOOP
Journal: Z Zellforsch Mikrosk Anat Date: 1957

4. Electrochemical aspects of physiological and pharmacological action in excitable cells. II. The action potential and excitation.

Authors: A M SHANES
Journal: Pharmacol Rev Date: 1958-06 Impact factor: 25.468

5. [Electron microscopic picture of an eosinophilic granulocyte].

Authors: W BARGMANN; A KNOOP
Journal: Z Zellforsch Mikrosk Anat Date: 1956

6. [Comparative electron microscopic research of pulmonary capillaries].

Authors: W BARGMANN; A KNOOP
Journal: Z Zellforsch Mikrosk Anat Date: 1956

7. The fine structure of nerve cell bodies and their myelin sheaths in the eighth nerve ganglion of the goldfish.

Authors: J ROSENBLUTH; S L PALAY
Journal: J Biophys Biochem Cytol Date: 1961-04

8. Improvements in epoxy resin embedding methods.

Authors: J H LUFT
Journal: J Biophys Biochem Cytol Date: 1961-02

9. The fine structure of neurons.

Authors: S L PALAY; G E PALADE
Journal: J Biophys Biochem Cytol Date: 1955-01

10. Electron microscopic study of demyelination in an experimentally induced lesion in adult cat spinal cord.

Authors: R P BUNGE; M B BUNGE
Journal: J Biophys Biochem Cytol Date: 1960-07

125 in total

1. ELECTRON-MICROSCOPIC HISTOCHEMISTRY OF CHOLINESTERASES IN THE RAT BRAIN.

Authors: S MORI; T MAEDA; N SHIMIZU
Journal: Z Zellforch Microsk Anat Histochem Date: 1964

2. THE FINE STRUCTURE OF CEREBRAL FLUID ACCUMULATION. III. EXTRACELLULAR SPREAD OF CRYPTOCOCCAL POLYSACCHARIDES IN THE ACUTE STAGE.

Authors: A HIRANO; H M ZIMMERMAN; S LEVINE
Journal: Am J Pathol Date: 1964-07 Impact factor: 4.307

3. THE ULTRASTRUCTURE OF THE INTRANUCLEAR RODLET IN CERTAIN NERVE CELLS.

Authors: K A SIEGESMUND; C R DUTTA; C A FOX
Journal: J Anat Date: 1964-01 Impact factor: 2.610

4. STAINING PROPERTIES OF LANTHANUM ON CELL MEMBRANES.

Authors: C F DOGGENWEILER; S FRENK
Journal: Proc Natl Acad Sci U S A Date: 1965-02 Impact factor: 11.205

5. [Recent results in the field of neurohistology: Nissl substance, cortical synapses, neuroglia and intercellular space].

Authors: P GLEES
Journal: Dtsch Z Nervenheilkd Date: 1963