Literature DB >> 14468121

Purification of staphylococcal alpha-hemolysin.

M A MADOFF, L WEINSTEIN.   

Abstract

Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914-918. 1962. - Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.

Entities:  

Keywords:  STAPHYLOCOCCUS; TOXINS AND ANTITOXINS/chemistry

Mesh：

Substances：

Year:  1962        PMID： 14468121      PMCID： PMC279374          DOI： 10.1128/jb.83.4.914-918.1962

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  A study of 435 strains of Staphylococcus pyogenes with reference to factors which may contribute to pathogenicity.

Authors:  C H LACK; D G WAILLING
Journal:  J Pathol Bacteriol       Date:  1954-10

2.  Starch-gel electrophoresis of anterior pituitary hormones.

Authors:  K A FERGUSON; A L WALLACE
Journal:  Nature       Date:  1961-05-13       Impact factor: 49.962

3.  The purification and concentration of influenza A virus (PR8) by a method of continuous extraction with glycine of a zinc-virus precipitate.

Authors:  W J HAUSLER; E C DICK
Journal:  J Infect Dis       Date:  1960 Sep-Oct       Impact factor: 5.226

4.  Studies on the preparation and isoelectric point of staphylococcal alpha-haemolysin.

Authors:  L O BUTLER
Journal:  Biochem J       Date:  1959-01       Impact factor: 3.857

5.  Serologic studies of the neuraminidases of Vibrio cholerae, Diplococcus pneumoniae and influenza virus.

Authors:  M A MADOFF; E H EYLAR; L WEINSTEIN
Journal:  J Immunol       Date:  1960-12       Impact factor: 5.422

6.  Purification of staphylococcal coagulase.

Authors:  H BLOBEL; D T BERMAN; J SIMON
Journal:  J Bacteriol       Date:  1960-06       Impact factor: 3.490

7.  Studies with staphylococcal toxins. V. Possible identification of alpha hemolysin with a proteolytic enzyme.

Authors:  J ROBINSON; F S THATCHER; J MONTFORD
Journal:  Can J Microbiol       Date:  1960-04       Impact factor: 2.419

8.  Purification of the toxin of Pasteurella pestis by continuous-flow paper electrophoresis.

Authors:  M L SPIVACK; A KARLER
Journal:  J Immunol       Date:  1958-06       Impact factor: 5.422

9.  [Purification of alpha staphylococcal toxin and toxoid].

Authors:  A Turpin; E H Relyveld; J Pillet; M Raynaud
Journal:  Ann Inst Pasteur (Paris)       Date:  1954-08

10.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157
  10 in total
  16 in total

Review 1.  The hemolysins of Staphylococcus aureus.

Authors:  G M Wiseman
Journal:  Bacteriol Rev       Date:  1975-12

2.  MECHANISM OF ACTION OF STAPHYLOCOCCAL ALPHA-HEMOLYSIN. I. SOME FACTORS INFLUENCING THE MEASUREMENT OF ALPHA-HEMOLYSIN.

Authors:  A A MARUCCI
Journal:  J Bacteriol       Date:  1963-12       Impact factor: 3.490

3.  ISOLATION AND CHARACTERIZATION OF STAPHYLOCOCCUS ALPHA-HEMOLYSIN.

Authors:  S KITAMURA; J SHELTON; A P THAL
Journal:  Ann Surg       Date:  1964-11       Impact factor: 12.969

4.  Effects of purified staphylococcal alpha toxin on the ultrastructure of human and rabbit erythrocytes.

Authors:  A S Klainer; T W Chang; L Weinstein
Journal:  Infect Immun       Date:  1972-05       Impact factor: 3.441

5.  Physical states of staphylococcal alpha-toxin.

Authors:  J P Arbuthnott; J H Freer; A W Bernheimer
Journal:  J Bacteriol       Date:  1967-10       Impact factor: 3.490

6.  Partial purification and characterization of the multiple molecular forms of staphylococcal clotting activity (coagulase).

Authors:  M W Reeves; M C Drummond; M Tager
Journal:  J Bacteriol       Date:  1981-12       Impact factor: 3.490

7.  Heat reactivation of the alpha-hemolytic, dermonecrotic, lethal activities of crude and purified staphylococcal alpha-toxin.

Authors:  M Manohar; S Kumar; R K Lindorfer
Journal:  J Bacteriol       Date:  1966-05       Impact factor: 3.490

8.  Heat stability and species range of purified staphylococcal alpha-toxin.

Authors:  L Z Cooper; M A Madoff; L Weinstein
Journal:  J Bacteriol       Date:  1966-05       Impact factor: 3.490

9.  Production, purification, and composition of staphylococcal alpha toxin.

Authors:  J R Coulter
Journal:  J Bacteriol       Date:  1966-12       Impact factor: 3.490

10.  Effect of purified staphylococal alpha toxin on active sodium transport and aerobic respiration in the isolated toad bladder.

Authors:  J J Rahal; M E Plaut; L Weinstein
Journal:  J Clin Invest       Date:  1968-07       Impact factor: 14.808
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