| Literature DB >> 144598 |
H Breer, S J Morris, V P Whittaker.
Abstract
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromagography on CPG-10-3000 porous glass beads. The synaptic vesicles have a buoyant density of 1.057 g/ml. The purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+, K+-stimulated ATPase activity. They contain a ouabaininsensitive, Na+, K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline.Entities:
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Year: 1977 PMID： 144598 DOI： 10.1111/j.1432-1033.1977.tb11884.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956