The vacuolating virus of monkeys. I. Isolation, growth characteristics, and inclusion body formation.

A vacuolating virus isolated from uninoculated patas monkey kidney cultures was found to be serologically identical with SV(40), a virus previously found in association with rhesus and cynomolgus monkeys. Detection of the patas virus was facilitated when patas cells were exposed to x-ray treatment. Rhesus monkey and human cells were relatively insusceptible to the virus, although it persisted in these cells for a long period of time. Distinct intranuclear inclusions were detected in infected patas cultures 2 to 3 days before cytoplasmic vacuoles were noticeable. Cultures previously infected with PA-57 virus did not affect yields of poliovirus, and doubly infected cells were easily distinguished in stained preparations.